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TF2H2 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - TF2H2 Antibody AP50741
    Western blot analysis of lysates from K562 cell line and human skeletal muscle tissue lysate(from left to right),using TF2H2 Antibody(AP50741). AP50741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q13888
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Calculated MW 44 KDa
Additional info
Gene ID 2966
Other Names General transcription factor IIH subunit 2, Basic transcription factor 2 44 kDa subunit, BTF2 p44, General transcription factor IIH polypeptide 2, TFIIH basal transcription factor complex p44 subunit, GTF2H2, BTF2P44
Dilution WB~~1:1000
Format Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions-20℃
Protein Information
Name GTF2H2
Synonyms BTF2P44
Function Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre- initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.
Cellular Location Nucleus.
Tissue Location Widely expressed, with higher expression in skeletal muscle.
Research Areas

BACKGROUND

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C- terminus is required for a successful escape of RNAP II form the promoter.

REFERENCES

Humbert S.,et al.EMBO J. 13:2393-2398(1994).
Zhang Q.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
van der Steege G.,et al.Eur. J. Hum. Genet. 3:87-95(1995).
Chen Q.,et al.Genomics 48:121-127(1998).
Kershnar E.,et al.J. Biol. Chem. 273:34444-34453(1998).

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