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ADIPOR2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ADIPOR2 Antibody (Center) AP5105C
    Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ADIPOR2 Antibody (Center) AP5105C
    Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - ADIPOR2 Antibody (Center) AP5105C
    Overlay histogram showing U-2 OS cells stained with AP5105c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5105c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ADIPOR2 Antibody (Center) AP5105C
    All lanes : Anti-ADIPOR2 Antibody (Center) at 1:2000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ADIPOR2 Antibody (Center) AP5105C
    Western blot analysis of ADIPOR2 Antibody (Center) (Cat. #AP5105c) in Hela cell line lysates (35ug/lane).ADIPOR2 (arrow) was detected using the purified Pab.
  • 3 - ADIPOR2 Antibody (Center) AP5105C
    Confocal immunofluorescent analysis of ADIPOR2 Antibody (Center) (Cat. #AP5105c) with Hela cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, FC, E
Primary Accession Q86V24
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43884 Da
Additional info
Gene ID 79602
Other Names Adiponectin receptor protein 2, Progestin and adipoQ receptor family member II, ADIPOR2, PAQR2
Target/Specificity This ADIPOR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-72 amino acids from the Central region of human ADIPOR2.
Dilution WB~~1:1000
FC~~1:25
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADIPOR2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADIPOR2 (HGNC:24041)
Function Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed:12802337, PubMed:25855295). Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).
Cellular Location Cell membrane; Multi-pass membrane protein Note=Localized to the cell membrane and intracellular organelles
Tissue Location Ubiquitous (PubMed:16044242). Highly expressed in skeletal muscle, liver and placenta (PubMed:12802337). Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung (PubMed:12802337)
Research Areas
Novel phytopeptide osmotin mimics preventive effects of adiponectin on vascular inflammation and atherosclerosis.
Author : Takahashi Y1,Watanabe R1,Sato Y1,Ozawa N1,Kojima M1,Watanabe-Kominato K1,Shirai R1,Sato K1,Hirano T2,Watanabe T3.
Metabolism. 2018 Feb 1. pii: S0026-0495(18)30016-7. doi: 10.1016/j.metabol.2018.01.010. [Epub ahead of print]
29410350

BACKGROUND

The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.

REFERENCES

Ferguson, J.F., et al. Am. J. Clin. Nutr. 91(3):794-801(2010) Halvatsiotis, I., et al. Cardiovasc Diabetol 9, 10 (2010) Yoshihara, K., et al. J Reprod Med 54 (11-12), 669-674 (2009)

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