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TFIIB Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - TFIIB Antibody AP53273
    Western blot detection of TFIIB in Jurkat,MCF7,COS7,3T3 and Hela cell lysates using TFIIB mouse mAb (1:500 diluted).Observed band size: 34KDa
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q00403
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Calculated MW 34 KDa
Additional Information
Gene ID 2959
Other Names General transcription factor IIB;General transcription factor TFIIB;gtf2b;RNA polymerase II transcription factor IIB;S300 II;S300-II;TF IIB;TF2B;TF2B_HUMAN;TFIIB;Transcription initiation factor IIB.
Dilution WB~~1:500
Format ascites
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name GTF2B
Synonyms TF2B, TFIIB
Function General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter- specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP- independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).
Cellular Location Nucleus. Chromosome. Note=Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171).
Tissue Location Expressed in the inner cell mass forming the embryoblast (PubMed:24441171). Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171)
Research Areas

BACKGROUND

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

REFERENCES

Ha I.,et al.Nature 352:689-695(1991).
Malik S.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:9553-9557(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Gregory S.G.,et al.Nature 441:315-321(2006).

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