注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   CD3d Antibody   

CD3d Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CD3d Antibody AP53292
    All lanes : Anti-CD3d Antibody at 1:500 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P04234
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 19 KDa
Additional info
Gene ID 915
Other Names T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD3d, CD3D, T3D
Dilution WB~~ 1:500
Format Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CD3D
Synonyms T3D
Function Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells
Research Areas

BACKGROUND

The CD3 complex mediates signal transduction.

REFERENCES

van den Elsen P.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:2944-2948(1986).
van den Elsen P.,et al.Nature 312:413-418(1984).
Tunnacliffe A.,et al.EMBO J. 5:1245-1252(1986).
Jin P.,et al.Genomics 83:566-571(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价