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Anti-CK19 Mouse mAb

Purified Mouse Monoclonal Antibody (Mab)

     
  • 2 - Anti-CK19 Mouse mAb AP53489
    Immunohistochemical analysis of KRT19 in Human appendix tissue sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1/200) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC
Primary Accession P08727
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monclonal Antibody
Isotype IgG1
Immunogen Synthetic peptide conjugated to KLH.
Purification Affinity Purification
Calculated MW 44kDa KDa
Additional Information
Gene ID 3880
Other Names Cytokeratin 19, Keratin type I cytoskeletal 19, 40 kDa keratin intermediate filament
Dilution IHC~~1:200
Format PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Protein Information
Name KRT19
Function Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue Location Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Research Areas

BACKGROUND

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

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