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Anti-CD45 Mouse mAb

Purified Mouse Monoclonal Antibody (Mab)

     
  • 2 - Anti-CD45 Mouse mAb AP53490
    Immunohistochemical analysis of CD45 in Human tonsil tissue sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1/400) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession P08575
Reactivity Human
Host Mouse
Clonality Monclonal Antibody
Isotype Mouse IgG1
Immunogen Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.
Purification Affinity Purification
Calculated MW 147.3kDa KDa
Antigen Region aa928-989
Additional Information
Gene ID 5788
Other Names LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Dilution IHC~~1:400
Format Purified antibody in PBS with 0.05% sodium azide
Storage Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Protein Information
Name PTPRC (HGNC:9666)
Synonyms CD45
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Membrane raft Note=Colocalized with DPP4 in membrane rafts
Tissue Location Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes
Research Areas

BACKGROUND

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

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