|Application ||WB, IHC|
|Immunogen||Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.|
|Calculated MW||147.3kDa KDa|
|Other Names||LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180|
|Format||Purified antibody in PBS with 0.05% sodium azide|
|Storage||Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.|
|Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Membrane raft Note=Colocalized with DPP4 in membrane rafts|
|Tissue Location||Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.