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>   首页   >   产品   >   一抗   >   其他   >   SARS virus PUP1 Antibody (N-term)   

SARS virus PUP1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
E
Primary Accession Q6VA99
Other Accession P59632, NP_828852
Reactivity SARS
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30903 Da
Additional info
Target/Specificity This SARS virus PUP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from aa 118-148 of SARS virus PUP1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSARS virus PUP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Q6VA99
Research Areas

BACKGROUND

The SARS-CoV genome contains five major open reading frames (ORFs) that encode the replicase polyprotein (R), the spike (S), envelope (E), and membrane (M) glycoproteins; and the nucleocapsid protein (N). Other proteins not falling into these categories have been termed PUPs (putative uncharacterized proteins) for their unknown structural or functional features and dissimilarity to those known sequences. However, it has been found that some of the PUPs matched the entries in the NCBI database. PUP1 is equivalent to ORF3 in Isolate Tor2. It receives 11 hits in GenBank through BLAST, two of which are putative transmembrane proteins. One is from Ralstonia solanacearum, cytochrome b-561, with 97 amino acids of PUP1 aligned, and the other is from Sinorhizobium meliloti, with 94 amino acids aligned. Sequence identities are 28% and 25%, respectively. Three putative transmembrane domains are located within PUP1.

REFERENCES

He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).
Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
Marra, M.A., et al., Science 300(5624):1399-1404 (2003).

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