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>   首页   >   产品   >   一抗   >   微生物学   >   SARS virus PUP5 Antibody (C-term)   

SARS virus PUP5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 文献引用 : 2
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
E
Primary Accession P59595
Reactivity SARS
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46025 Da
Additional info
Gene ID 1489678
Other Names Nucleoprotein, Nucleocapsid protein, NC, Protein N, N
Target/Specificity This SARS virus PUP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393~422 amino acids from the C-terminus region of SARS nucleocapsid protein.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSARS virus PUP5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name N {ECO:0000255|HAMAP-Rule:MF_04096}
Function Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Cellular Location Virion {ECO:0000255|HAMAP-Rule:MF_04096, ECO:0000269|PubMed:17210170, ECO:0000269|PubMed:19106108}. Host endoplasmic reticulum-Golgi intermediate compartment {ECO:0000255|HAMAP-Rule:MF_04096, ECO:0000269|PubMed:17210170} Host Golgi apparatus {ECO:0000255|HAMAP-Rule:MF_04096, ECO:0000269|PubMed:17210170}. Host cytoplasm, host perinuclear region. Note=Located inside the virion, complexed with the viral RNA. Probably associates with ER- derived membranes where it participates in viral RNA synthesis and virus budding. {ECO:0000255|HAMAP-Rule:MF_04096}
Research Areas
Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus.
Author : Yamate M1, Yamashita M, Goto T, Tsuji S, Li YG, Warachit J, Yunoki M, Ikuta K.
Microbes Infect. 2005 Dec;7(15):1530-40. Epub 2005 Jul 6.
16269264
Susceptibility of human and rat neural cell lines to infection by SARS-coronavirus.
Author : Yamashita M1, Yamate M, Li GM, Ikuta K.
Biochem Biophys Res Commun. 2005 Aug 19;334(1):79-85.
15992768

BACKGROUND

An outbreak of atypical pneumonia, referred to as severe acute respiratory syndrome (SARS) and first identified in Guangdong Province, China, has spread to several countries. The severity of this disease is such that the mortality rate appears to be ~3 to 6%. A number of laboratories worldwidehave undertaken the identification of the causative agent. The National Microbiology Laboratory in Canada obtained the Tor2 isolate from a patient in Toronto, and succeeded in growing a coronavirus-like agent in African Green Monkey Kidney (Vero E6) cells. This coronavirus has been named publicly by the World Health Organization and member laboratories as ?SARS virus? The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein. The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis. The site of viral attachment to the host cell resides within the S protein. Oligomeric spike (S) glycoproteins extend from SARS membranes. These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments. Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.

REFERENCES

He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).
Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
Krokhin, O., et al., Mol Cell Proteomics 2(5):346-356 (2003).

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