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SIRT3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SIRT3 Antibody (C-term) AP6242a
    All lanes : Anti-SIRT3 Antibody (C-term) at 1:2000 dilution Lane 1: human kidney lysate Lane 2: human liver lysate Lane 3: mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SIRT3 Antibody (C-term) AP6242a
    Western blot analysis of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody (C-term)(Cat. #AP6242a). AP6242a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - SIRT3 Antibody (C-term) AP6242a
    SIRT3 Antibody (C-term) (Cat. #AP6242a) western blot analysis in KG-1 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
  • 1 - SIRT3 Antibody (C-term) AP6242a
    SIRT3 Antibody (C-term) (Cat. #AP6242a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SIRT3 antibody detected the SIRT3 protein (arrow).
  • 14 - SIRT3 Antibody (C-term) AP6242a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9NTG7
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43573 Da
Additional info
Gene ID 23410
Other Names NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3, 351-, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, SIRT3, SIR2L3
Target/Specificity This SIRT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the C-terminal region of human SIRT3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIRT3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIRT3
Synonyms SIR2L3
Function NAD-dependent protein deacetylase (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090, PubMed:19535340). Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues (PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:23283301, PubMed:24121500, PubMed:24252090). Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO (PubMed:16788062, PubMed:18680753, PubMed:24121500, PubMed:24252090, PubMed:19535340). Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels (PubMed:18794531). In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription (PubMed:23283301).
Cellular Location Mitochondrion matrix
Tissue Location Widely expressed.
Research Areas
Sirtuin3 protects aged human mesenchymal stem cells against oxidative stress and enhances efficacy of cell therapy for ischaemic heart diseases.
Author : Zhang DY1,2,Zhang CF1,2,Fu BC1,2,Sun L1,2,Wang XQ1,2,Chen W1,2,Liu W2,Liu KY1,2,Du GQ2,Ma CY1,2,Jiang SL1,2,Li RK3,4,Tian H1,2.
J Cell Mol Med. 2018 Aug 9. doi: 10.1111/jcmm.13821. [Epub ahead of print]
30091830
Activation of AMPK-SIRT3 Signaling is Chondroprotective by Preserving Mitochondrial DNA Integrity and Function.
Author : Chen LY1,Wang Y2,Terkeltaub R3,Liu-Bryan R4.
Osteoarthritis Cartilage. 2018 Jul 19. pii: S1063-4584(18)31373-6. doi: 10.1016/j.joca.2018.07.004. [Epub ahead of print]
30031925
Exercise in the Prevention and Management of Oxidative Stress-Linked Diseases.
Author : Venditti P1,Zhang Y2,Radák Z3,Magalhães J4.
Oxid Med Cell Longev. 2018 Apr 26;2018:7643721. doi: 10.1155/2018/7643721. eCollection 2018.
29854095
Short-Duration Swimming Exercise after Myocardial Infarction Attenuates Cardiac Dysfunction and Regulates Mitochondrial Quality Control in Aged Mice.
Author : Zhao D1,Sun Y2,Tan Y1,Zhang Z2,Hou Z3,Gao C4,Feng P1,Zhang X3,Yi W1,Gao F3.
Oxid Med Cell Longev. 2018 Apr 11;2018:4079041. doi: 10.1155/2018/4079041. eCollection 2018.
29849892
Decreased Sirtuin Deacetylase Activity in LRRK2 G2019S iPSC-Derived Dopaminergic Neurons.
Author : Schwab AJ1,Sison SL1,Meade MR1,Broniowska KA2,Corbett JA2,Ebert AD3.
Stem Cell Reports. 2017 Oct 31. pii: S2213-6711(17)30465-4. doi: 10.1016/j.stemcr.2017.10.010. [Epub ahead of print]
29129681
Localization of sirtuins (SIRT1-7) in the aged mouse inner ear.
Author : Takumida M1, Takumida H2, Katagiri Y1, Anniko M3.
Acta Otolaryngol. 2015 Oct 15:1-12. [Epub ahead of print]
26472659
Activation of the aryl hydrocarbon receptor sensitizes mice to nonalcoholic steatohepatitis by deactivating mitochondrial sirtuin deacetylase Sirt3.
Author : He J, Hu B, Shi X, Weidert ER, Lu P, Xu M, Huang M, Kelley EE, Xie W.
Mol Cell Biol. 2013 May;33(10):2047-55. doi: 10.1128/MCB.01658-12. Epub 2013 Mar 18.
23508103
Receptor-interacting protein (RIP) and Sirtuin-3 (SIRT3) are on opposite sides of anoikis and tumorigenesis.
Author : Kamarajan P, Alhazzazi TY, Danciu T, D'silva NJ, Verdin E, Kapila YL.
Cancer. 2012 Dec 1;118(23):5800-10. doi: 10.1002/cncr.27655. Epub 2012 Jun 6.
22674009
Integration of β-catenin, sirtuin, and FOXO signaling protects from mutant huntingtin toxicity.
Author : Parker JA1, Vazquez-Manrique RP, Tourette C, Farina F, Offner N, Mukhopadhyay A, Orfila AM, Darbois A, Menet S, Tissenbaum HA, Neri C.
J Neurosci. 2012 Sep 5;32(36):12630-40. doi: 10.1523/JNEUROSCI.0277-12.2012.
22956852
PPARα-LXR as a novel metabolostatic signalling axis in skeletal muscle that acts to optimize substrate selection in response to nutrient status.
Author : Caton PW, Holness MJ, Bishop-Bailey D, Sugden MC.
Biochem J. 2011 Aug 1;437(3):521-30. doi: 10.1042/BJ20110702.
21609322
Sirtuin-3 (SIRT3), a novel potential therapeutic target for oral cancer.
Author : Alhazzazi TY, Kamarajan P, Joo N, Huang JY, Verdin E, D'Silva NJ, Kapila YL.
Cancer. 2011 Apr 15;117(8):1670-8. doi: 10.1002/cncr.25676. Epub 2010 Nov 29.
21472714
FoxO1 mediates an autofeedback loop regulating SIRT1 expression.
Author : Xiong S, Salazar G, Patrushev N, Alexander RW.
J Biol Chem. 2011 Feb 18;286(7):5289-99. doi: 10.1074/jbc.M110.163667. Epub 2010 Dec 13.
21149440
Exogenous NAD blocks cardiac hypertrophic response via activation of the SIRT3-LKB1-AMP-activated kinase pathway.
Author : Pillai VB1, Sundaresan NR, Kim G, Gupta M, Rajamohan SB, Pillai JB, Samant S, Ravindra PV, Isbatan A, Gupta MP.
J Biol Chem. 2010 Jan 29;285(5):3133-44. doi: 10.1074/jbc.M109.077271. Epub 2009 Nov 24.
19940131
Sirt3 blocks the cardiac hypertrophic response by augmenting Foxo3a-dependent antioxidant defense mechanisms in mice.
Author : Sundaresan NR, Gupta M, Kim G, Rajamohan SB, Isbatan A, Gupta MP.
J Clin Invest. 2009 Sep;119(9):2758-71. doi: 10.1172/JCI39162. Epub 2009 Aug 3.
19652361
SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70.
Author : Sundaresan NR, Samant SA, Pillai VB, Rajamohan SB, Gupta MP.
Mol Cell Biol. 2008 Oct;28(20):6384-401. doi: 10.1128/MCB.00426-08. Epub 2008 Aug 18.
18710944

BACKGROUND

SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

REFERENCES

Hirschey, M.D., et al. Nature 464(7285):121-125(2010) Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010) Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)

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