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SPHK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - SPHK1 Antibody (Center) AP7237C
    AP7237c staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - SPHK1 Antibody (Center) AP7237C
    AP7237c staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - SPHK1 Antibody (Center) AP7237C
    AP7237c staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - SPHK1 Antibody (Center) AP7237C
    All lanes : Anti-SPHK1 Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SPHK1 Antibody (Center) AP7237C
    Anti-SPHK1 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SPHK1 Antibody (Center) AP7237C
    Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301)(Cat. #AP7237c). AP7237c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - SPHK1 Antibody (Center) AP7237C
    Western blot analysis of SPHK1 (arrow) using rabbit polyclonal SPHK1 Antibody (R301) (Cat.#AP7237c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPHK1 gene.
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  • 文献引用 : 25
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9NYA1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 8877
Other Names Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Target/Specificity This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPHK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPHK1
Synonyms SPHK, SPK
Function Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N- dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Cellular Location Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner
Tissue Location Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle
Research Areas
Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease.
Author : Lee JY1,2,3,Han SH1,2,3,Park MH1,2,3,Baek B1,4,Song IS5,Choi MK6,Takuwa Y7,Ryu H8,9,Kim SH10,He X11,Schuchman EH11,Bae JS12,13,14,Jin HK15,16.
Nat Commun. 2018 Apr 16;9(1):1479. doi: 10.1038/s41467-018-03674-2.
29662056
Predictive and prognostic value of sphingosine kinase 1 expression in patients with invasive ductal carcinoma of the breast.
Author : Do SI1,Kim HS2,Kim K1,Lee H1,Do IG1,Kim DH1,Chae SW1,Sohn JH1.
Am J Transl Res. 2017 Dec 15;9(12):5684-5695. eCollection 2017.
29312521
Insulin-like growth factor receptor and sphingosine kinase are prognostic and therapeutic targets in breast cancer.
Author : Ochnik AM1,2,Baxter RC3.
BMC Cancer. 2017 Dec 5;17(1):820. doi: 10.1186/s12885-017-3809-0.
29207959
Predictive Value of Sphingosine Kinase 1 Expression in Papillary Thyroid Carcinoma.
Author : Do SI1,Kim HS2,Kim K1,Lee H1,Do IG1,Kim DH1,Chae SW1,Sohn JH1.
Anticancer Res. 2017 Oct;37(10):5399-5405.
28982849
Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain.
Author : Song DD1,Zhang TT1,Chen JL1,Xia YF1,Qin ZH1,Waeber C2,3,Sheng R1.
Cell Death Dis. 2017 Jul 6;8(7):e2912. doi: 10.1038/cddis.2017.289.
28682313
Overexpression of sphingosine kinase 1 is predictive of poor prognosis in human breast cancer.
Author : Zhu YJ1,You H2,Tan JX3,Li F3,Qiu Z1,Li HZ1,Huang HY1,Zheng K1,3,Ren GS1,3.
Oncol Lett. 2017 Jul;14(1):63-72. doi: 10.3892/ol.2017.6134. Epub 2017 May 8.
28693136
Sphingosine kinase 1 expression enhances colon tumor growth.
Author : Furuya H1,2,Shimizu Y3,4,5,Tamashiro PM3,Iino K3,Bielawski J6,Chan OTM4,Pagano I7,Kawamori T8,9,10.
J Transl Med. 2017 Jun 6;15(1):120. doi: 10.1186/s12967-017-1220-x.
28583134
Sphingosine-1-phosphate is involved in the occlusive arteriopathy of pulmonary arterial hypertension.
Author : Gairhe S1,Joshi SR2,Bastola MM3,McLendon JM2,Oka M1,Fagan KA1,McMurtry IF1.
Pulm Circ. 2016 Sep;6(3):369-80. doi: 10.1086/687766.
27683614
Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer.
Author : Matula K1, Collie-Duguid E1, Murray G1,2, Parikh K1, Grabsch H3, Tan P4, Lalwani S1, Garau R1, Ong Y1, Bain G1,5, Smith AD<
BMC Cancer. 2015 Oct 22;15:762. doi: 10.1186/s12885-015-1718-7.
26493335
Sphingosine kinase 1 mediates neuroinflammation following cerebral ischemia.
Author : Zheng S1, Wei S2, Wang X3, Xu Y2, Xiao Y2, Liu H2, Jia J4, Cheng J5.
Exp Neurol. 2015 Mar 19. pii: S0014-4886(15)00080-1. doi: 10.1016/j.expneurol.2015.03.012. [Epub ahead of print]
25797575
The apoptotic mechanism of action of the sphingosine kinase 1 selective inhibitor SKI-178 in human acute myeloid leukemia cell lines.
Author : Dick TE1, Hengst JA1, Fox TE1, Colledge AL1, Kale VP1, Sung SS1, Sharma A1, Amin S1, Loughran TP Jr1, Kester M1, Wang HG1, Yun JK
J Pharmacol Exp Ther. 2015 Mar;352(3):494-508. doi: 10.1124/jpet.114.219659. Epub 2015 Jan 6.
25563902
SphK1 confers resistance to apoptosis in gastric cancer cells by downregulating Bim via stimulating Akt/FoxO3a signaling.
Author : Xiong H1, Wang J2, Guan H3, Wu J4, Xu R1, Wang M1, Rong X1, Huang K1, Huang J1, Liao Q1, Fu Y1, Yuan J4.
Oncol Rep. 2014 Oct;32(4):1369-73. doi: 10.3892/or.2014.3391. Epub 2014 Aug 7.
25109605
Isoflurane attenuates blood-brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice.
Author : Altay O1, Suzuki H, Hasegawa Y, Caner B, Krafft PR, Fujii M, Tang J, Zhang JH.
Stroke. 2012 Sep;43(9):2513-6. doi: 10.1161/STROKEAHA.112.661728. Epub 2012 Jul 5.
22773559
Sphingosine kinase-1 enhances resistance to apoptosis through activation of PI3K/Akt/NF-κB pathway in human non-small cell lung cancer.
Author : Song L, Xiong H, Li J, Liao W, Wang L, Wu J, Li M.
Clin Cancer Res. 2011 Apr 1;17(7):1839-49. doi: 10.1158/1078-0432.CCR-10-0720. Epub 2011 Feb 15.
21325072
Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury.
Author : Kim M, Park SW, Kim M, D'Agati VD, Lee HT.
Anesthesiology. 2011 Feb;114(2):363-73. doi: 10.1097/ALN.0b013e3182070c3a.
21245730
Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions.
Author : Fischer I, Alliod C, Martinier N, Newcombe J, Brana C, Pouly S.
PLoS One. 2011;6(8):e23905. doi: 10.1371/journal.pone.0023905. Epub 2011 Aug 24.
21887342
Isoflurane activates intestinal sphingosine kinase to protect against bilateral nephrectomy-induced liver and intestine dysfunction.
Author : Kim M, Park SW, Kim M, D'Agati VD, Lee HT.
Am J Physiol Renal Physiol. 2011 Jan;300(1):F167-76. doi: 10.1152/ajprenal.00467.2010. Epub 2010 Oct 20.
20962114
Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy.
Author : Liu G, Zheng H, Zhang Z, Wu Z, Xiong H, Li J, Song L.
BMC Cancer. 2010 Sep 16;10:495. doi: 10.1186/1471-2407-10-495.
20846391
Sphingosine kinase 1 regulates the expression of proinflammatory cytokines and nitric oxide in activated microglia.
Author : Nayak D, Huo Y, Kwang WX, Pushparaj PN, Kumar SD, Ling EA, Dheen ST.
Neuroscience. 2010 Mar 10;166(1):132-44. doi: 10.1016/j.neuroscience.2009.12.020. Epub 2009 Dec 28.
20036321
Sphingosine-1-phosphate elicits receptor-dependent calcium signaling in retinal amacrine cells.
Author : Crousillac S, Colonna J, McMains E, Dewey JS, Gleason E.
J Neurophysiol. 2009 Dec;102(6):3295-309. doi: 10.1152/jn.00119.2009. Epub 2009 Sep 23.
19776367
Differential regulation of sphingosine kinases 1 and 2 in lung injury.
Author : Wadgaonkar R, Patel V, Grinkina N, Romano C, Liu J, Zhao Y, Sammani S, Garcia JG, Natarajan V.
Am J Physiol Lung Cell Mol Physiol. 2009 Apr;296(4):L603-13. doi: 10.1152/ajplung.90357.2008. Epub 2009 Jan 23.
19168577
Sphingosine kinase 1 is associated with gastric cancer progression and poor survival of patients.
Author : Li W, Yu CP, Xia JT, Zhang L, Weng GX, Zheng HQ, Kong QL, Hu LJ, Zeng MS, Zeng YX, Li M, Li J, Song LB.
Clin Cancer Res. 2009 Feb 15;15(4):1393-9. doi: 10.1158/1078-0432.CCR-08-1158.
19228740
Clinical significance of sphingosine kinase-1 expression in human astrocytomas progression and overall patient survival.
Author : Li J, Guan HY, Gong LY, Song LB, Zhang N, Wu J, Yuan J, Zheng YJ, Huang ZS, Li M.
Clin Cancer Res. 2008 Nov 1;14(21):6996-7003. doi: 10.1158/1078-0432.CCR-08-0754.
18980995
Activation of sphingosine kinase-1 mediates inhibition of vascular smooth muscle cell apoptosis by hyperglycemia.
Author : You B, Ren A, Yan G, Sun J.
Diabetes. 2007 May;56(5):1445-53. Epub 2007 Feb 26.
17325258
FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1.
Author : Sun J, Yan G, Ren A, You B, Liao JK.
Circ Res. 2006 Sep 1;99(5):468-76. Epub 2006 Aug 3.
16888242

BACKGROUND

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

REFERENCES

Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000). Melendez, A.J., et al., Gene 251(1):19-26 (2000). Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).

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