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MAPK3 Antibody (Y204)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MAPK3 Antibody (Y204) AP7251e
    Western blot analysis of MAPK3 (arrow) using rabbit polyclonal MAPK3 Antibody (Y204) (RB13301). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK3 gene (Lane 2) (Origene Technologies).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P27361
Other Accession P39745, P21708, Q63844, P26696, P63086, P63085, P28482, P46196, P40417
Reactivity Human
Predicted Drosophila, Bovine, Mouse, Rat, Xenopus, C.Elegans
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43136 Da
Additional info
Gene ID 5595
Other Names Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3
Target/Specificity This MAPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from human MAPK3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAPK3 Antibody (Y204) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAPK3
Synonyms ERK1, PRKM3
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Cellular Location Cytoplasm. Nucleus. Membrane, caveola {ECO:0000250|UniProtKB:P21708}. Note=Autophosphorylation at Thr- 207 promotes nuclear localization
Research Areas
Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells.
Author : Tetsunaga T, Nishida K, Furumatsu T, Naruse K, Hirohata S, Yoshida A, Saito T, Ozaki T.
Osteoarthritis Cartilage. 2011 Feb;19(2):222-32. doi: 10.1016/j.joca.2010.11.004. Epub 2010 Nov 19.
21094261

BACKGROUND

MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.

REFERENCES

Munshi, H.G., et al., J. Biol. Chem. 279(37):39042-39050 (2004).
Mukherjee, S., et al., Infect. Immun. 72(9):5274-5282 (2004).
Sebkova, L., et al., Infect. Immun. 72(9):5019-5026 (2004).
Huang, H.M., et al., Biochem. Biophys. Res. Commun. 320(4):1247-1252 (2004).
Mizuno, S., et al., Am. J. Respir. Cell Mol. Biol. 31(2):184-192 (2004).

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