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ERBB4 Antibody(N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ERBB4 Antibody(N-term) AP7631a
    Western blot analysis of anti-ErbB4 Pab (Cat. #AP7631a) in HL60 cell lysate. ErbB4 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • 14 - ERBB4 Antibody(N-term) AP7631a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q15303
Other Accession Q62956, Q61527
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 146808 Da
Additional info
Gene ID 2066
Other Names Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4, ERBB4 intracellular domain, 4ICD, E4ICD, s80HER4, ERBB4, HER4
Target/Specificity This ERBB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of human ERBB4.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsERBB4 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ERBB4
Synonyms HER4
Function Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=In response to NRG1 treatment, the activated receptor is internalized
Tissue Location Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
Research Areas
miR‑1273g‑3p promotes proliferation, migration and invasion of LoVo cells via cannabinoid receptor 1 through activation of ERBB4/PIK3R3/mTOR/S6K2 signaling pathway.
Author : Li M1,Qian X2,Zhu M3,Li A3,Fang M1,Zhu Y4,Zhang J3.
Mol Med Rep. 2018 Mar;17(3):4619-4626. doi: 10.3892/mmr.2018.8397. Epub 2018 Jan 9.
29328379
Resistance to receptor-blocking therapies primes tumors as targets for HER3-homing nanobiologics.
Author : Sims JD1,Taguiam JM1,Alonso-Valenteen F1,Markman J1,Agadjanian H1,Chu D1,Lubow J1,Abrol R1,Srinivas D1,Jain A1,Han B1,Qu Y1,Mirzadehgan P1,Hwang JY1,Rentsendorj A1,Chung A1,Lester J1,Karlan BY1,Gray HB2,Gross Z3,Giuliano A1,Cui X1,Medina-Kauwe LK4.
J Control Release. 2017 Dec 27. pii: S0168-3659(17)31094-5. doi: 10.1016/j.jconrel.2017.12.024. [Epub ahead of print]
29288681
Uncoupling of EGFR-RAS signaling and nuclear localization of YBX1 in colorectal cancer.
Author : Roßner F1,2, Gieseler C1, Morkel M1, Royer HD3, Rivera M4, Blaker H1,2, Dietel M1, Schafer R1,2, Sers C1,2.
Oncogenesis. 2016 Jan 18;5:e187. doi: 10.1038/oncsis.2015.51.
26779809
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Author : Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T.
Circ Res. 2009 Aug 28;105(5):500-10. doi: 10.1161/CIRCRESAHA.109.193870. Epub 2009 Jul 30.
19644050

BACKGROUND

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

REFERENCES

Cheng, Q.C., et al., J. Biol. Chem. 278(40):38421-38427 (2003). Komuro, A., et al., J. Biol. Chem. 278(35):33334-33341 (2003). Williams, E.E., et al., Cancer Lett. 192(1):67-74 (2003). Thomas, C.Y., et al., Int. J. Cancer 104(1):19-27 (2003). Ni, C.Y., et al., J. Biol. Chem. 278(7):4561-4565 (2003).

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