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ALDH1A3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ALDH1A3 Antibody (N-term) AP7847a
    ALDH1A3 Antibody (N-term) (Cat. #AP7847a) western blot analysis in 293,Y79 cell line lysates (35ug/lane).This demonstrates the ALDH1A3 antibody detected the ALDH1A3 protein (arrow).
  • 14 - ALDH1A3 Antibody (N-term) AP7847a
    Formalin-fixed and paraffin-embedded human kideny tissue reacted with ALDH1A3 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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  • 文献引用 : 9
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P47895
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 220
Other Names Aldehyde dehydrogenase family 1 member A3, Aldehyde dehydrogenase 6, Retinaldehyde dehydrogenase 3, RALDH-3, RalDH3, ALDH1A3, ALDH6
Target/Specificity This ALDH1A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ALDH1A3.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH1A3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH1A3
Synonyms ALDH6 {ECO:0000303|PubMed:7698756}
Function NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid (PubMed:27759097). Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde (PubMed:27759097). Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q9JHW9}.
Tissue Location Expressed at low levels in many tissues and at higher levels in salivary gland, stomach, and kidney
Research Areas
ALDH1A3 is epigenetically regulated during melanocyte transformation and is a target for melanoma treatment.
Author : Pérez-Alea M1,McGrail K1,Sánchez-Redondo S1,Ferrer B1,2,Fournet G3,Cortés J4,5,Muñoz E5,Hernandez-Losa J2,Tenbaum S6,Martin G7,Costello R8,Ceylan I7,Garcia-Patos V1,9,Recio JA1.
Oncogene. 2017 Jun 5. doi: 10.1038/onc.2017.160. [Epub ahead of print]
28581514
Therapeutic potential of the metabolic modulator phenformin in targeting the stem cell compartment in melanoma.
Author : Petrachi T1,Romagnani A1,Albini A2,Longo C3,Argenziano G3,4,Grisendi G5,Dominici M5,Ciarrocchi A1,Dallaglio K1.
Oncotarget. 2017 Jan 24;8(4):6914-6928. doi: 10.18632/oncotarget.14321.
28036292
Induced Expression of Cancer Stem Cell Markers ALDH1A3 and Sox-2 in Hierarchical Reconstitution of Apoptosis-resistant Human Breast Cancer Cells.
Author : Kashii-Magaribuchi K1,Takeuchi R2,Haisa Y2,Sakamoto A2,Itoh A2,Izawa Y1,Isa M2,Fukuzawa M2,Murakami M2,Takahashi R3.
Acta Histochem Cytochem. 2016 Nov 1;49(5):149-158. Epub 2016 Oct 26.
27917009
Aldh1 Expression and Activity Increase During Tumor Evolution in Sarcoma Cancer Stem Cell Populations.
Author : Martinez-Cruzado L1, Tornin J1, Santos L1, Rodriguez A1, García-Castro J2, Morís F3, Rodriguez R1.
Sci Rep. 2016 Jun 13;6:27878. doi: 10.1038/srep27878.
27292183
ALDH Enzyme Expression Is Independent of the Spermatogenic Cycle and Their Inhibition Causes Misregulation of Murine Spermatogenic Processes.
Author : Kent T, Arnold SL, Fasnacht R, Rowsey R, Mitchell D, Hogarth CA, Isoherranen N, Griswold MD.
Biol Reprod. 2015 Dec 2. pii: biolreprod.115.131458. [Epub ahead of print]
26632609
Down-regulation of ALDH1A3, CD44 or MDR1 sensitizes resistant cancer cells to FAK autophosphorylation inhibitor Y15.
Author : Golubovskaya V1, O\'Brien S, Ho B, Heffler M, Conroy J, Hu Q, Wang D, Liu S, Cance WG.
J Cancer Res Clin Oncol. 2015 Feb 6. [Epub ahead of print]
25656374
Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.
Author : Arnold SL1, Kent T2, Hogarth CA2, Schlatt S3, Prasad B1, Haenisch M4, Walsh T5, Muller CH5, Griswold MD2, Amory JK6, Isoherranen N1.
J Lipid Res. 2015 Feb;56(2):342-57. doi: 10.1194/jlr.M054718. Epub 2014 Dec 11.
25502770
Essential role of aldehyde dehydrogenase 1A3 for the maintenance of non-small cell lung cancer stem cells is associated with the STAT3 pathway.
Author : Shao C1, Sullivan JP2, Girard L3, Augustyn A1, Yenerall P1, Rodriguez-Canales J4, Liu H4, Behrens C4, Shay JW5, Wistuba II4, Minna JD6
Clin Cancer Res. 2014 Aug 1;20(15):4154-66. doi: 10.1158/1078-0432.CCR-13-3292. Epub 2014 Jun 6.
24907115
Cellular level classification of breast cancer through proteomic markers using nanochannel array sensors.
Author : Selvam AP1, Wong J, Flanagan K, Prasad S.
Nanomedicine (Lond). 2013 Nov 18. [Epub ahead of print]
24237262

BACKGROUND

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme ALDH1A3 uses retinal as a substrate, either in a free or cellular retinol-binding protein form.

REFERENCES

Rexer,B.N., Cancer Res. 61 (19), 7065-7070 (2001)
Yoshida,A., Eur. J. Biochem. 251 (3), 549-557 (1998)

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