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MYLK3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MYLK3 Antibody (N-term) AP7965A
    Western blot analysis of anti-MYLK3 Antibody (N-term) (Cat. #AP7965a) in A375 cell line lysates (35ug/lane). MYLK3 (arrow) was detected using the purified Pab.
  • 1 - MYLK3 Antibody (N-term) AP7965A
    Western blot analysis of anti-MYLK3 Antibody (N-term) (Cat. #AP7965a) in mouse heart tissue lysates (35ug/lane). MYLK3(arrow) was detected using the purified Pab.
  • 14 - MYLK3 Antibody (N-term) AP7965A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 14 - MYLK3 Antibody (N-term) AP7965A
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MYLK3 antibody (N-term) (Cat.#AP7965a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q32MK0
Other Accession Q96DV1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 88393 Da
Additional info
Gene ID 91807
Other Names Myosin light chain kinase 3, Cardiac-MyBP-C-associated Ca/CaM kinase, Cardiac-MLCK, MYLK3, MLCK
Target/Specificity This MYLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human MYLK3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMYLK3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYLK3
Synonyms MLCK
Function Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).
Cellular Location Cytoplasm.
Tissue Location Restricted to heart.
Research Areas
Cardiac-Directed Expression of Adenylyl Cyclase Catalytic Domain Reverses Cardiac Dysfunction Caused by Sustained Beta-Adrenergic Receptor Stimulation.
Author : Gao MH1,Lai NC1,Giamouridis D1,Kim YC1,Tan Z1,Guo T1,Dillmann WH1,Suarez J1,Hammond HK1.
JACC Basic Transl Sci. 2016 Dec;1(7):617-629. doi: 10.1016/j.jacbts.2016.08.004.
28670631
Interferon Stimulated Gene 15 upregulation precedes the development of blood brain barrier disruption and cerebral edema after traumatic brain injury in young mice.
Author : Rossi JL1, Todd T, Daniels Z, Bazan NG, Belayev L.
J Neurotrauma. 2015 Feb 10. [Epub ahead of print]
25669448
Albumin causes increased myosin light chain kinase expression in astrocytes via p38 mitogen-activated protein kinase.
Author : Rossi JL, Ralay Ranaivo H, Patel F, Chrzaszcz M, Venkatesan C, Wainwright MS.
J Neurosci Res. 2011 Jun;89(6):852-61. doi: 10.1002/jnr.22600. Epub 2011 Feb 24.
21360574

BACKGROUND

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

REFERENCES

Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

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