- 文献引用 : 2
|Application ||WB, IHC-P, E|
|Calculated MW||61036 Da|
|Other Names||Phosphatidylinositol 4-phosphate 5-kinase type-1 beta, PIP5K1-beta, PtdIns(4)P-5-kinase 1 beta, Phosphatidylinositol 4-phosphate 5-kinase type I beta, PIP5KIbeta, Protein STM-7, Type I phosphatidylinositol 4-phosphate 5-kinase beta, PIP5K1B, STM7|
|Target/Specificity||This PIP5K1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PIP5K1B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PIP5K1B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).|
|Cellular Location||Endomembrane system. Note=Associated with membranes.|
|Tissue Location||Detected in heart, pancreas, brain, kidney, skeletal muscle and lung.|
Author : Qin Y, Li L, Pan W, Wu D.
J Biol Chem. 2009 Aug 21;284(34):22544-8. doi: 10.1074/jbc.M109.014399. Epub 2009 Jun 26.
Author : Bolomini-Vittori M, Montresor A, Giagulli C, Staunton D, Rossi B, Martinello M, Constantin G, Laudanna C.
Nat Immunol. 2009 Feb;10(2):185-94. doi: 10.1038/ni.1691. Epub 2009 Jan 11.
Provided below are standard protocols that you may find useful for product applications.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
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