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PACSIN2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - PACSIN2 Antibody (C-term) AP8088b
    Immunohistochemical analysis of paraffin-embedded H. stomach section using PACSIN2 Antibody (C-term)(Cat#AP8088b). AP8088b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - PACSIN2 Antibody (C-term) AP8088b
    PACSIN2 Antibody (S357) (Cat. #AP8088b) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
  • 14 - PACSIN2 Antibody (C-term) AP8088b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9UNF0
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 11252
Other Names Protein kinase C and casein kinase substrate in neurons protein 2, Syndapin-2, Syndapin-II, PACSIN2
Target/Specificity This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.
Dilution IHC-P~~1:50~100
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPACSIN2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PACSIN2
Function Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle- mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome Recycling endosome membrane. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Membrane, caveola. Note=Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1
Tissue Location Widely expressed.
Research Areas
A disease-associated frameshift mutation in caveolin-1 disrupts caveolae formation and function through introduction of a de novo ER retention signal.
Author : Copeland CA1,Han B1,Tiwari A1,Austin ED2,Loyd JE3,West JD3,Kenworthy AK4,5,6,7.
Mol Biol Cell. 2017 Sep 13. pii: mbc.E17-06-0421. doi: 10.1091/mbc.E17-06-0421. [Epub ahead of print]
28904206
Clostridium difficile Toxin A Undergoes Clathrin-Independent, PACSIN2-Dependent Endocytosis.
Author : Chandrasekaran R1,Kenworthy AK2,3,4,Lacy DB1,4,5.
PLoS Pathog. 2016 Dec 12;12(12):e1006070. doi: 10.1371/journal.ppat.1006070. eCollection 2016.
27942025
Characterization of a caveolin-1 mutation associated with both PAH and congenital generalized lipodystrophy.
Author : Han B1, Copeland CA1, Kawano Y2, Rosenzweig EB2, Austin ED3, Shahmirzadi L4, Tang S4, Raghunathan K2, Chung WK2, Kenworthy AK5.
Traffic. 2016 Sep 26. doi: 10.1111/tra.12452. [Epub ahead of print]
27717241
EHD3 is Required for Tubular Recycling Endosome Stabilization and an Asparagine-Glutamic Acid Residue Pair within its EH Domain Dictates its Selective Binding to NPF Peptides.
Author : Bahl K1, Xie S1, Spagnol G1, Sorgen PL1, Naslavsky N1, Caplan S2.
J Biol Chem. 2016 May 4. pii: jbc.M116.716407. [Epub ahead of print]
27189942
Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development.
Author : Bhattacharyya S1,2, Rainey MA2, Arya P3,2, Dutta S4, George M2, Storck MD2, McComb RD1, Muirhead D1, Todd GL3, Gould K3, Da
Sci Rep. 2016 Feb 17;6:20727. doi: 10.1038/srep20727.
26884322
Differential roles of C-terminal Eps15 homology domain proteins as vesiculators and tubulators of recycling endosomes.
Author : Cai B1, Giridharan SS, Zhang J, Saxena S, Bahl K, Schmidt JA, Sorgen PL, Guo W, Naslavsky N, Caplan S.
J Biol Chem. 2013 Oct 18;288(42):30172-80. doi: 10.1074/jbc.M113.488627. Epub 2013 Sep 9.
24019528
Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis.
Author : Giridharan SS1, Cai B, Vitale N, Naslavsky N, Caplan S.
Mol Biol Cell. 2013 Jun;24(11):1776-90, S1-15. doi: 10.1091/mbc.E13-01-0026. Epub 2013 Apr 17.
23596323
The F-BAR protein PACSIN2 regulates epidermal growth factor receptor internalization.
Author : de Kreuk BJ, Anthony EC, Geerts D, Hordijk PL.
J Biol Chem. 2012 Dec 21;287(52):43438-53. doi: 10.1074/jbc.M112.391078. Epub 2012 Nov 5.
23129763
Pacsin 2 is recruited to caveolae and functions in caveolar biogenesis.
Author : Hansen CG, Howard G, Nichols BJ.
J Cell Sci. 2011 Aug 15;124(Pt 16):2777-85. doi: 10.1242/jcs.084319.
21807942
The F-BAR domain protein PACSIN2 associates with Rac1 and regulates cell spreading and migration.
Author : de Kreuk BJ, Nethe M, Fernandez-Borja M, Anthony EC, Hensbergen PJ, Deelder AM, Plomann M, Hordijk PL.
J Cell Sci. 2011 Jul 15;124(Pt 14):2375-88. doi: 10.1242/jcs.080630. Epub 2011 Jun 21.
21693584

BACKGROUND

PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.

REFERENCES

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Wiemann, S., et al., Genome Res. 11(3):422-435 (2001). Ritter, B., et al., FEBS Lett. 454(3):356-362 (1999). Dunham, I., et al., Nature 402(6761):489-495 (1999).

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