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SCP2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SCP2 Antibody (Center) AP8639C
    All lanes : Anti-SCP2 Antibody (Center) at 1:500-2000 dilution Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - SCP2 Antibody (Center) AP8639C
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SCP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - SCP2 Antibody (Center) AP8639C
    Flow cytometric analysis of HepG2 cells using SCP2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P22307
Other Accession P11915, O62742, P32020, P07857
Reactivity Human, Mouse, Rat
Predicted Bovine, Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 58994 Da
Additional info
Gene ID 6342
Other Names Non-specific lipid-transfer protein, NSL-TP, Propanoyl-CoA C-acyltransferase, SCP-chi, SCPX, Sterol carrier protein 2, SCP-2, Sterol carrier protein X, SCP-X, SCP2
Target/Specificity This SCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-385 amino acids from the Central region of human SCP2.
Dilution WB~~1:500-2000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSCP2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SCP2
Function Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis.
Cellular Location Cytoplasm. Mitochondrion. Note=Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues Isoform SCP2: Mitochondrion.
Tissue Location Liver, fibroblasts, and placenta.
Research Areas

BACKGROUND

SCP2 mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. It may play a role in regulating steroidogenesis.

REFERENCES

Wu, Y.B. et al. J Biol Chem. 2009 January 2; 284(1): 640?48.
Baker,M.E., et.al., DNA Cell Biol. 10 (9), 695-698 (1991)
Vila,A., et.al., Biochemistry 43 (39), 12592-12605 (2004)

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