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ATP5I Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ATP5I Antibody (C-term) AW5003
    Western blot analysis of lysates from mouse liver, heart tissue and A431 cell line (from left to right), using ATP5I Antibody (C-term)(Cat. #AW5003). AW5003 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.
  • 14 - ATP5I Antibody (C-term) AW5003
    Immunohistochemical analysis of paraffin-embedded H. liver section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ATP5I Antibody (C-term) AW5003
    Immunohistochemical analysis of paraffin-embedded M. heart section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ATP5I Antibody (C-term) AW5003
    Immunohistochemical analysis of paraffin-embedded H. kidney section using ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - ATP5I Antibody (C-term) AW5003
    Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 3 - ATP5I Antibody (C-term) AW5003
    Fluorescent image of Hela cells stained with ATP5I Antibody (C-term)(Cat#AW5003). AW5003 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, IHC-P, WB
Primary Accession P56385
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW H=8;M=8 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 521
Antigen Region 55-89
Other Names ATP synthase subunit e, mitochondrial, ATPase subunit e, ATP5I, ATP5K
Dilution WB~~1:1000
IHC-P~~1:25
IF~~1:25
Target/Specificity This ATP5I antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-89 amino acids from the C-terminal region of human ATP5I.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP5I Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP5ME (HGNC:846)
Function Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.
Cellular Location Mitochondrion. Mitochondrion inner membrane.
Research Areas

BACKGROUND

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane.

REFERENCES

Fujiwara T.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Xu G.,et al.Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

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