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RPL6 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - RPL6 Antibody (N-term) AW5024
    Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right),using RPL6 Antibody (N-term)(Cat. #AW5024). AW5024 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - RPL6 Antibody (N-term) AW5024
    Flow cytometric analysis of Jurkat cells using RPL6 Antibody (N-term)(green, Cat#AW5024) compared to an isotype control of rabbit IgG(blue). AW5024 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - RPL6 Antibody (N-term) AW5024
    Fluorescent image of HeLa cells stained with RPL6 Antibody (N-term)(Cat#AW5024). AW5024 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - RPL6 Antibody (N-term) AW5024
    Immunohistochemical analysis of paraffin-embedded H. gastric carcinoma section using RPL6 Antibody (N-term)(Cat#NA). NA was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB
Primary Accession Q02878
Reactivity Human
Host Rabbit
Clonality polyclonal
Calculated MW H=33 KDa
Isotype Rabbit Ig
Antigen Source HUMAN
Additional Information
Gene ID 6128
Antigen Region 2-35
Other Names 60S ribosomal protein L6, Neoplasm-related protein C140, Tax-responsive enhancer element-binding protein 107, TaxREB107, RPL6, TXREB1
Dilution WB~~1:1000
FC~~1:25
IF~~1:25
IHC-P~~1:25
Target/Specificity This RPL6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-35amino acids from the N-terminal region of human RPL6.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPL6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPL6
Synonyms TXREB1
Function Component of the large ribosomal subunit.
Cellular Location Cytoplasm, cytosol. Cytoplasm. Rough endoplasmic reticulum {ECO:0000250|UniProtKB:Q2YGT9} Note=Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). {ECO:0000250|UniProtKB:Q2YGT9, ECO:0000269|PubMed:25957688}

BACKGROUND

Specifically binds to domain C of the Tax-responsive enhancer element in the long terminal repeat of HTLV-I.

REFERENCES

Zaman G.J.R.,et al.Nucleic Acids Res. 21:1673-1673(1993).
Morita T.,et al.AIDS Res. Hum. Retroviruses 9:115-121(1993).
Kenmochi N.,et al.J. Hum. Genet. 45:290-293(2000).
Ohta K.,et al.J. Mol. Endocrinol. 12:85-92(1994).
Odintsova T.I.,et al.J. Protein Chem. 22:249-258(2003).

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