|Application ||IHC-P, IF, WB|
|Other Accession||P62193, P62192|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||H=49;M=49;Rat=49 KDa|
|Other Names||26S protease regulatory subunit 4;26S protease regulatory subunit 4; 26S protease regulatory subunit 4; 26S proteasome AAA-ATPase subunit RPT2; 26S protease regulatory subunit 4; Proteasome 26S subunit ATPase 1|
|Target/Specificity||This PRS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of human PRS4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRS4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.|
|Cellular Location||Cytoplasm. Nucleus. Membrane; Lipid-anchor|
Provided below are standard protocols that you may find useful for product applications.
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Dubiel W., et al. J. Biol. Chem. 267:22699-22702(1992).
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Matilla A., et al. Hum. Mol. Genet. 10:2821-2831(2001).
Brill L.M., et al. Anal. Chem. 76:2763-2772(2004).
Katiyar S., et al. Proc. Natl. Acad. Sci. U.S.A. 101:13774-13779(2004).