|Application ||FC, IHC-P, WB|
|Calculated MW||H=57,52,50;M=57;Rat=57 KDa|
|Other Names||HMGCS2; Hydroxymethylglutaryl-CoA synthase, mitochondrial; 3-hydroxy-3-methylglutaryl coenzyme A synthase|
|Target/Specificity||This HMGCS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 478-508 amino acids from the C-terminal region of human HMGCS2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HMGCS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.|
|Tissue Location||Expression in liver is 200-fold higher than in any other tissue. Low expression in colon, kidney, testis, and pancreas. Very low expression in heart and skeletal muscle. Not detected in brain. The relative expression of isoform 3 (at mRNA level) is highest in heart (70%) and skeletal muscle (60%)|
Provided below are standard protocols that you may find useful for product applications.
HMGCS2 belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting.
Lu,Y., et.al., J. Lipid Res. 49 (12), 2582-2589 (2008)