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SSPN Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Western blot analysis of lysates from HL-60,K562,MDA-MB-453 cell line (from left to right), using SSPN Antibody (N-term)(Cat. #AW5200). AW5200 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q14714
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	H=27,14 KDa
Isotype	Rabbit Ig
Antigen Source	Human

Additional Information
Antigen Region	6-34
Other Names	SSPN;KRAG; Sarcospan; Sarcospan; K-ras oncogene-associated protein; Sarcospan; Kirsten-ras-associated protein
Dilution	WB~~1:1000
Target/Specificity	This SSPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human SSPN.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SSPN Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SSPN
Synonyms	KRAG
Function	Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.
Cellular Location	Cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein Note=Also found in myotendinous junctions and in the postsynaptic membrane of neuromuscular junctions.
Tissue Location	Isoform 1 is expressed exclusively in heart and skeletal muscle. Isoform 2 is expressed in heart, skeletal muscle, thymus, prostate, testis, ovary, small intestine, colon and spleen EMBL; AF016028; AAC61660.2; -; mRNA EMBL; AL136756; CAB66690.1; -; mRNA EMBL; AK092925; BAG52629.1; -; mRNA EMBL; AC022509; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC055720; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC062299; AAH62299.1; -; mRNA EMBL; X89105; CAA61479.1; -; mRNA CCDS; CCDS44850.1; -. [Q14714-3] CCDS; CCDS8707.1; -. [Q14714-1] PIR; S57712; S57712 RefSeq; NP_001129295.1; NM_001135823.1. [Q14714-3] RefSeq; NP_005077.2; NM_005086.4. [Q14714-1] RefSeq; XP_011519155.1; XM_011520853.2. [Q14714-3] RefSeq; XP_011519156.1; XM_011520854.2. [Q14714-3] RefSeq; XP_011519157.1; XM_011520855.2. [Q14714-3] UniGene; Hs.183428; - UniGene; Hs.736914; - ProteinModelPortal; Q14714; - BioGrid; 113755; 1 CORUM; Q14714; - IntAct; Q14714; 2 MINT; Q14714; - STRING; 9606.ENSP00000242729; - iPTMnet; Q14714; - PhosphoSitePlus; Q14714; - SwissPalm; Q14714; - BioMuta; SSPN; - DMDM; 13431900; - jPOST; Q14714; - PaxDb; Q14714; - PeptideAtlas; Q14714; - PRIDE; Q14714; - ProteomicsDB; 60142; - ProteomicsDB; 60143; -. [Q14714-2] DNASU; 8082; - Ensembl; ENST00000242729; ENSP00000242729; ENSG00000123096. [Q14714-1] Ensembl; ENST00000422622; ENSP00000396087; ENSG00000123096. [Q14714-3] Ensembl; ENST00000540266; ENSP00000442893; ENSG00000123096. [Q14714-3] GeneID; 8082; - KEGG; hsa:8082; - UCSC; uc001rhd.4; human. [Q14714-1] CTD; 8082; - DisGeNET; 8082; - EuPathDB; HostDB:ENSG00000123096.11; - GeneCards; SSPN; - HGNC; HGNC:11322; SSPN HPA; HPA066432; - MIM; 601599; gene neXtProt; NX_Q14714; - OpenTargets; ENSG00000123096; - PharmGKB; PA36146; - eggNOG; ENOG410IK33; Eukaryota eggNOG; ENOG4111WM7; LUCA GeneTree; ENSGT00390000007747; - HOGENOM; HOG000065718; - HOVERGEN; HBG005016; - InParanoid; Q14714; - KO; K22194; - OMA; YVGVRMC; - OrthoDB; 629563at2759; - PhylomeDB; Q14714; - TreeFam; TF332100; - SIGNOR; Q14714; - ChiTaRS; SSPN; human GenomeRNAi; 8082; - PRO; PR:Q14714; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000123096; Expressed in 237 organ(s), highest expression level in myometrium ExpressionAtlas; Q14714; baseline and differential Genevisible; Q14714; HS GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW GO; GO:0016010; C:dystrophin-associated glycoprotein complex; TAS:ProtInc GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell GO; GO:0042383; C:sarcolemma; IEA:UniProtKB-SubCell GO; GO:0030133; C:transport vesicle; IDA:LIFEdb GO; GO:0007155; P:cell adhesion; TAS:ProtInc GO; GO:0006936; P:muscle contraction; TAS:ProtInc InterPro; IPR007237; CD20-like InterPro; IPR030429; Sarcospan PANTHER; PTHR15260; PTHR15260; 1 Pfam; PF04103; CD20; 1 2: Evidence at transcript level; Alternative splicing; Cell junction; Cell membrane; Complete proteome; Membrane; Polymorphism; Postsynaptic cell membrane; Reference proteome; Synapse; Transmembrane; Transmembrane helix CHAIN 1 243 Sarcospan /FTId=PRO_0000072226 TOPO_DOM 1 53 Cytoplasmic. TRANSMEM 54 74 Helical. TOPO_DOM 75 86 Extracellular. TRANSMEM 87 107 Helical. TOPO_DOM 108 122 Cytoplasmic. TRANSMEM 123 143 Helical. TOPO_DOM 144 193 Extracellular. TRANSMEM 194 214 Helical. TOPO_DOM 215 243 Cytoplasmic. VAR_SEQ 1 103 Missing (in isoform 3) /FTId=VSP_046351 VAR_SEQ 108 218 Missing (in isoform 2) /FTId=VSP_004431 VARIANT 186 186 S -> N (in dbSNP:rs12313670) /FTId=VAR_051384 VARIANT 228 228 V -> I (in dbSNP:rs12313736) /FTId=VAR_051385 CONFLICT 13 13 Missing (in Ref. 1). SEQUENCE 243 AA; 26618 MW; 0D6B24B9848253F9 CRC64; MGKNKQPRGQ QRQGGPPAAD AAGPDDMEPK KGTGAPKECG EEEPRTCCGC RFPLLLALLQ LALGIAVTVV GFLMASISSS LLVRDTPFWA GIIVCLVAYL GLFMLCVSYQ VDERTCIQFS MKLLYFLLSA LGLTVCVLAV AFAAHHYSQL TQFTCETTLD SCQCKLPSSE PLSRTFVYRD VTDCTSVTGT FKLFLLIQMI LNLVCGLVCL LACFVMWKHR YQVFYVGVRI CSLTASEGPQ QKI

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Component of the dystrophin-glycoprotein complex (DGC), a complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. Preferentially associates with the sarcoglycan subcomplex of the DGC.

REFERENCES

Crosbie R.H., et al. J. Biol. Chem. 272:31221-31224(1997).
Crosbie R.H., et al. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Wiemann S., et al. Genome Res. 11:422-435(2001).
Heighway J., et al. Genomics 35:207-214(1996).

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¥ 3,250.00

¥ 1,600.00

Cat# AW5200

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SSPN Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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