|Application ||FC, IHC-P, WB|
|Other Accession||P15429, Q1KYT0, P21550, Q3ZC09|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||H=47,44,42;M=47;Rat=47 KDa|
|Other Names||ENO3; Beta-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; Skeletal muscle enolase|
|Target/Specificity||This ENOB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-264 amino acids from the Central region of human ENOB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ENOB Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Appears to have a function in striated muscle development and regeneration.|
|Cellular Location||Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity)|
|Tissue Location||The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons|
Provided below are standard protocols that you may find useful for product applications.
ENOB is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.
Aurino,S., Acta Myol 27, 90-97 (2008)
Giallongo,A., Eur. J. Biochem. 214 (2), 367-374 (1993)