|Application ||IHC-P, WB|
|Calculated MW||H=11 KDa|
|Other Names||COX6A2; COX6A; COX6AH; Cytochrome c oxidase subunit 6A2, mitochondrial; Cytochrome c oxidase polypeptide VIa-heart; Cytochrome c oxidase subunit VIA-muscle|
|Target/Specificity||This COX6A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human COX6A2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||COX6A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.|
|Cellular Location||Mitochondrion inner membrane.|
|Tissue Location||Expressed specifically in heart and muscle.|
Provided below are standard protocols that you may find useful for product applications.
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.
Bachman, N.J., et al. Genomics 42(1):146-151(1997)
Lanfranchi, G., et al. Genome Res. 6(1):35-42(1996)