|Application Notes||Ready-to-use mouse spleen tissue lysate is prepared as a positive control for separation by SDS-PAGE and subsequent western blot analysis. Heat lysate to 95°C for 5 minutes and rapidly cool. The recommended loading volume per lane is 2~10 ul depending on the size format of your gel.|
|Format||Whole lysate is stored in 1 X SDS 0.3% beta-mercaptoethanol sample buffer.|
|Storage||Store vial at -70°C or colder. For extended storage, aliquot contents to minimize freeze/thaw cycles. Storage at -20°C will be stable for three months, storage at-70°C will be stable for one year.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
Provided below are standard protocols that you may find useful for product applications.