Anti-STAT5 (pY694/699) Antibody

Rabbit polyclonal antibody to STAT5 (pY694/699)

Western blot analysis of STAT5 (pY694/699) expression in K562 (A) whole cell lysates.
Immunohistochemical analysis of STAT5 (pY694/699) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P42229, P51692
Other Accession	P42230, P42232
Reactivity	Human, Mouse, Rat, Pig, Chicken, Bovine, SARS
Host	Rabbit
Clonality	Polyclonal
Calculated MW	90647 Da

Additional Information
Gene ID	6776
Other Names	STAT5A; STAT5; Signal transducer and activator of transcription 5A; STAT5B; Signal transducer and activator of transcription 5B
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human STAT5 (pY694/699). The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	STAT5A
Synonyms	STAT5
Function	Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL- induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location	Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.