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Myc Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - Myc Tag Antibody AM1007a
    Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Myc Tag Antibody AM1007a
    Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(Cat. #AM1007A). AM1007A was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
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  • 文献引用 : 16
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 9E10
Calculated MW 48 kDa KDa
Additional info
Other Names Tag from c-Myc protein
Target/Specificity KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Dilution WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyc Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
MAP6 interacts with Tctex1 and Cav 2.2/N-type calcium channels to regulate calcium signaling in neurons.
Author : Brocard J1,2,Dufour F1,2,Gory-Fauré S1,2,Arnoult C3,4,5,Bosc C1,2,Denarier E1,2,6,Peris L1,2,Saoudi Y1,2,De Waard M7,8,9,Andrieux A1,2,6.
Eur J Neurosci. 2017 Nov 2. doi: 10.1111/ejn.13766. [Epub ahead of print]
29094416
The Joubert Syndrome Protein Inpp5e Controls Ciliogenesis by Regulating Phosphoinositides at the Apical Membrane.
Author : Xu W1, Jin M1, Hu R1, Wang H1, Zhang F1, Yuan S2, Cao Y3.
J Am Soc Nephrol. 2016 Jul 8. pii: ASN.2015080906. [Epub ahead of print]
27401686
N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability.
Author : Chang CF1, Hsu LS2,3, Weng CY4, Chen CK5, Wang SY6, Chou YH7, Liu YY8, Yuan ZX9, Huang WY10, Lin H11, Chen YH12, Tsai JN Int J Mol Sci. 2016 Jun 14;17(6). pii: E937.
27314333
Characterization of DWARF14 Genes in Populus.
Author : Zheng K1,2, Wang X1,2, Weighill DA1,3, Guo HB4, Xie M1, Yang Y1, Yang J1,5, Wang S2, Jacobson DA1,3,<
Sci Rep. 2016 Feb 15;6:21593. doi: 10.1038/srep21593.
26875827
DELLA Proteins Interact with FLC to Repress the Flowering Transition.
Author : Li M1, An F1, Li W1, Ma M1, Feng Y1, Zhang X1, Guo H1.
J Integr Plant Biol. 2015 Nov 20. doi: 10.1111/jipb.12451. [Epub ahead of print]
26584710
Synergistic and Independent Actions of Multiple Terminal Nucleotidyl Transferases in the 3\' Tailing of Small RNAs in Arabidopsis.
Author : Wang X1, Zhang S2, Dou Y2, Zhang C2, Chen X3, Yu B2, Ren G4.
PLoS Genet. 2015 Apr 30;11(4):e1005091. doi: 10.1371/journal.pgen.1005091. eCollection 2015.
25928341
A small GTPase‑like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3.
Author : Hu X, Yu J, Zhou X, Li Z, Xia Y, Luo Z, Wu Y.
Mol Med Rep. 2014 Jan;9(1):173-9. doi: 10.3892/mmr.2013.1766. Epub 2013 Oct 30.
24172987
Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies.
Author : Liu Q1, Yan Q, Liu Y, Hong F, Sun Z, Shi L, Huang Y, Fang Y.
Plant Physiol. 2013 Sep;163(1):108-17. doi: 10.1104/pp.113.219071. Epub 2013 Jul 25.
23886622
A zebrafish model of congenital disorders of glycosylation with phosphomannose isomerase deficiency reveals an early opportunity for corrective mannose supplementation.
Author : Chu J1, Mir A, Gao N, Rosa S, Monson C, Sharma V, Steet R, Freeze HH, Lehrman MA, Sadler KC.
Dis Model Mech. 2013 Jan;6(1):95-105. doi: 10.1242/dmm.010116. Epub 2012 Aug 16.
22899857
UHRF1 phosphorylation by cyclin A2/cyclin-dependent kinase 2 is required for zebrafish embryogenesis.
Author : Chu J, Loughlin EA, Gaur NA, SenBanerjee S, Jacob V, Monson C, Kent B, Oranu A, Ding Y, Ukomadu C, Sadler KC.
Mol Biol Cell. 2012 Jan;23(1):59-70. doi: 10.1091/mbc.E11-06-0487. Epub 2011 Nov 9.
22072796
Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation.
Author : Satow R1, Shitashige M, Jigami T, Honda K, Ono M, Hirohashi S, Yamada T.
J Biol Chem. 2010 Aug 20;285(34):26289-94. doi: 10.1074/jbc.M109.090597. Epub 2010 Jun 21.
20566648
Receptor activator of NF-kappaB (RANK) ligand induces ectodomain shedding of RANK in murine RAW264.7 macrophages.
Author : Hakozaki A, Yoda M, Tohmonda T, Furukawa M, Hikata T, Uchikawa S, Takaishi H, Matsumoto M, Chiba K, Horiuchi K, Toyama Y.
J Immunol. 2010 Mar 1;184(5):2442-8. doi: 10.4049/jimmunol.0901188. Epub 2010 Jan 29.
20118276
Mutations in Arabidopsis fatty acid amide hydrolase reveal that catalytic activity influences growth but not sensitivity to abscisic acid or pathogens.
Author : Kim SC, Kang L, Nagaraj S, Blancaflor EB, Mysore KS, Chapman KD.
J Biol Chem. 2009 Dec 4;284(49):34065-74. doi: 10.1074/jbc.M109.059022. Epub 2009 Sep 30.
19801664
Effect of low-fat diet on development of prostate cancer and Akt phosphorylation in the Hi-Myc transgenic mouse model.
Author : Kobayashi N1, Barnard RJ, Said J, Hong-Gonzalez J, Corman DM, Ku M, Doan NB, Gui D, Elashoff D, Cohen P, Aronson WJ.
Cancer Res. 2008 Apr 15;68(8):3066-73. doi: 10.1158/0008-5472.CAN-07-5616.
18413778
Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1.
Author : Zemskov EA, Mikhailenko I, Strickland DK, Belkin AM.
J Cell Sci. 2007 Sep 15;120(Pt 18):3188-99. Epub 2007 Aug 21.
17711877
Identification of a novel recognition sequence for fibronectin within the NH2-terminal beta-sandwich domain of tissue transglutaminase.
Author : Hang J, Zemskov EA, Lorand L, Belkin AM.
J Biol Chem. 2005 Jun 24;280(25):23675-83. Epub 2005 Apr 22.
15849356

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.

REFERENCES

Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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