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TBB1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - TBB1 Antibody AM1013A
    All lanes : Anti-TBB1 Antibody at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - TBB1 Antibody AM1013A
    Western blot analysis of anti-TBB1 Antibody (Cat. #AM1013a) in mouse brain tissue, Y79, CEM and 293 lysates (35μg/lane). TBB1 (arrow) was detected using the purified Mab.(1μg/ml)
  • 14 - TBB1 Antibody AM1013A
    Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - TBB1 Antibody AM1013A
    Confocal immunofluorescent analysis of TBB1 Antibody (Cat#AM1013a) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q9H4B7
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 86CT4.4.4
Calculated MW 50327 Da
Additional info
Gene ID 81027
Other Names Tubulin beta-1 chain, TUBB1
Target/Specificity This monoclonal antibody is generated from mice immunized with a recombinant protein human TBB1.
Dilution WB~~1:100~500
IHC-P~~1:10~50
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB1
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Cellular Location Cytoplasm, cytoskeleton
Tissue Location Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins
Research Areas

BACKGROUND

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.

REFERENCES

Study of 18 functional hemostatic polymorphisms in mucocutaneous bleeding disorders. Antón AI, et al. Ann Hematol, 2010 Nov. PMID 20532885.
Natural product derivative Bis(4-fluorobenzyl)trisulfide inhibits tumor growth by modification of beta-tubulin at Cys 12 and suppression of microtubule dynamics. Xu W, et al. Mol Cancer Ther, 2009 Dec. PMID 19996274.
Genotype-phenotype relationship for six common polymorphisms in genes affecting platelet function from 286 healthy subjects and 160 patients with mucocutaneous bleeding of unknown cause. Martínez C, et al. Br J Haematol, 2009 Jun. PMID 19388931.
TUBB1 Q43P polymorphism does not protect against acute coronary syndrome and premature myocardial infarction. Navarro-Núñez L, et al. Thromb Haemost, 2008 Dec. PMID 19132255.
Roles of beta-tubulin residues Ala428 and Thr429 in microtubule formation in vivo. Joe PA, et al. J Biol Chem, 2009 Feb 13. PMID 19074767.

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