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|Application ||WB, E|
|Other Names||Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L|
|Target/Specificity||Purified His-tagged APG7 protein was used to produced this monoclonal antibody.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATG7 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).|
|Cellular Location||Cytoplasm. Preautophagosomal structure. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme|
|Tissue Location||Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.|
Author : Chiu HC, Kulp SK, Soni S, Wang D, Gunn JS, Schlesinger LS, Chen CS.
Antimicrob Agents Chemother. 2009 Dec;53(12):5236-44. doi: 10.1128/AAC.00555-09. Epub 2009 Oct 5.
Provided below are standard protocols that you may find useful for product applications.
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.
Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744.
Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Zhao Y, et al. Nat Cell Biol, 2010 Jul. PMID 20543840.
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Atg7 deficiency increases resistance of MCF-7 human breast cancer cells to photodynamic therapy. Xue LY, et al. Autophagy, 2010 Feb. PMID 20083906.
Proteasome inhibitors activate autophagy as a cytoprotective response in human prostate cancer cells. Zhu K, et al. Oncogene, 2010 Jan 21. PMID 19881538.