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HGF Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - HGF Antibody AM2064B
    Anti-Human HGF C-term at 1:4000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HGF Antibody AM2064B
    All lanes : Anti-Human HGF C-term at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P14210
Other Accession NP_000592.3
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 489CT6.12.6
Calculated MW 83134 Da
Additional info
Gene ID 3082
Other Names Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, HGF, HPTA
Target/Specificity This HGF antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from human HGF.
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HGF
Synonyms HPTA
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Research Areas

BACKGROUND

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq].

REFERENCES

Shi, E., et al. Anesthesiology 113(5):1109-1117(2010)
Hoot, K.E., et al. J. Clin. Invest. 120(10):3606-3616(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Togawa, A., et al. Biochem. Biophys. Res. Commun. 400(2):271-277(2010)
Li, F., et al. J. Biol. Chem. 285(36):27673-27685(2010)

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