RILPL1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

RILPL1 Antibody (C-term) (Cat. #AP10297b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the RILPL1 antibody detected the RILPL1 protein (arrow).
RILPL1 antibody (C-term) (Cat. #AP10297b) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RILPL1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q5EBL4
Other Accession	Q0IHE5, Q9JJC6, Q17QG3, NP_847884.2
Reactivity	Human, Mouse
Predicted	Bovine, Xenopus
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig
Calculated MW	47108 Da

Additional info
Gene ID	353116
Other Names	RILP-like protein 1, Rab-interacting lysosomal-like protein 1, RILPL1, RLP1
Target/Specificity	This RILPL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-366 amino acids from the C-terminal region of human RILPL1.
Dilution	WB~~1:1000 IHC-P~~1:50~100
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RILPL1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RILPL1
Synonyms	RLP1
Function	Plays a role in the regulation of cell shape and polarity. Plays a role in cellular protein transport, including protein transport away from primary cilia. Neuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus. Competes with SIAH1 for binding GAPDH (By similarity). Does not regulate lysosomal morphology and distribution.
Cellular Location	Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium
Tissue Location	Widely expressed. Expressed at lower level in liver and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Flow Cytomety Protocol

Cell Culture Protocol

Wang, T., et al. Mol. Biol. Cell 15(2):815-826(2004)

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Cat# AP10297b

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