CST9 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

CST9 Antibody (C-term) (Cat. #AP11059b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CST9 antibody detected the CST9 protein (arrow).
CST9 antibody (C-term) (Cat. #AP11059b) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CST9 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CST9 Antibody (C-term) (Cat. #AP11059b) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q5W186
Other Accession	NP_001008693.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	18135 Da
Antigen Region	131-159 aa

Additional Information
Gene ID	128822
Other Names	Cystatin-9, Cystatin-like molecule, CST9, CLM
Target/Specificity	This CST9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the C-terminal region of human CST9.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CST9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CST9
Function	May be involved in testis development (By similarity). May play a role in hematopoietic differentiation or inflammation (PubMed:12535658). Has immunomodulatory and antimicrobial functions against Francisella tularensis, a Gram-negative bacteria (PubMed:23922243).
Cellular Location	Secreted. Note=May be targeted through the Golgi via the secretory pathway
Tissue Location	Expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:12535658). Not expressed in brain (PubMed:12535658). Expressed in epididymis, kidney, testis, spinal cord, and thymus with a strong expression in epididymis and kidney and a weak expression in the spinal cord and thymus (PubMed:20565543)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.