癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CKAP4 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 实验流程
- 背景知识
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q07065 |
| Other Accession | NP_006816.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 66022 Da |
| Antigen Region | 574-602 aa |
| Gene ID | 10970 |
|---|---|
| Other Names | Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4 |
| Target/Specificity | This CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 574-602 amino acids from the C-terminal region of human CKAP4. |
| Dilution | IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CKAP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CKAP4 |
|---|---|
| Function | Mediates the anchoring of the endoplasmic reticulum to microtubules. |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region Note=Translocates to the perinuclear region upon APF-stimulation |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.
REFERENCES
Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010)
Tomkova, H., et al. Eur J Dermatol 20(3):411-413(2010)
Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009)
Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
Bates, S.R., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L658-L669 (2008) :
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Anonymous
2016-08-24 09:20:44
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Species tested
Human
Application tested
WB
Organization tested
H460,293T,U87,HepG2,Hela,BxPC-3,NIH/3T3,Brain(M),Brain(R)
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Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose
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