癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PIAS3 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9Y6X2 |
| Other Accession | O70260, O54714 |
| Reactivity | Human, Rat, Mouse |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 68017 Da |
| Antigen Region | 588-619 aa |
| Gene ID | 10401 |
|---|---|
| Other Names | E3 SUMO-protein ligase PIAS3, 632-, Protein inhibitor of activated STAT protein 3, PIAS3 |
| Target/Specificity | This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588-619 amino acids of human PIAS3. |
| Dilution | IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PIAS3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PIAS3 |
|---|---|
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:O54714}. Nucleus {ECO:0000250|UniProtKB:O54714}. Nucleus speckle {ECO:0000250|UniProtKB:O54714}. Note=Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules. {ECO:0000250|UniProtKB:O54714} |
| Tissue Location | Widely expressed.. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
REFERENCES
Nojiri, S., et al., Biochem. Biophys. Res. Commun. 314(1):97-103 (2004). Long, J., et al., Proc. Natl. Acad. Sci. U.S.A. 101(1):99-104 (2004). Cheng, J., et al., Leuk. Res. 28(1):71-82 (2004). Yamamoto, T., et al., Biochem. Biophys. Res. Commun. 306(2):610-615 (2003). Ueki, N., et al., J. Hum. Genet. 44(3):193-196 (1999).
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