UBN2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-UBN2 Antibody (N-term) at 1:500 dilution Lane 1: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 170kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-UBN2 Antibody (N-term) at 1:1000 dilution Lane 1: Ramos whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 146 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
UBN2 Antibody (N-term) (Cat. #AP13523a) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the UBN2 antibody detected the UBN2 protein (arrow).
UBN2 Antibody (N-term) (Cat. #AP13523a) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the UBN2 antibody detected the UBN2 protein (arrow).
UBN2 Antibody (N-term) (Cat. #AP13523a) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q6ZU65
Other Accession	NP_775840.3
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	146089 Da
Antigen Region	171-200 aa

Additional Information
Gene ID	254048
Other Names	Ubinuclein-2, UBN2, KIAA2030
Target/Specificity	This UBN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the N-terminal region of human UBN2.
Dilution	WB~~1:1000 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	UBN2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.