ASPG Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing HepG2 cells stained with AP13630c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP13630c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-ASPG Antibody (Center) at 1:1000 dilution Lane 1: Human heart lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, E
Primary Accession	Q86U10
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	374569
Other Names	60 kDa lysophospholipase, L-asparaginase, L-asparagine amidohydrolase, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, ASPG, C14orf76
Target/Specificity	This ASPG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human ASPG.
Dilution	FC~~1:25 WB~~1:1000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ASPG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ASPG
Synonyms	C14orf76
Function	Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Flow Cytomety Protocol

Cell Culture Protocol

ASPG exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

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Cat# AP13630c

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