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ASPG Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - ASPG Antibody (Center) AP13630c
    Overlay histogram showing HepG2 cells stained with AP13630c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP13630c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ASPG Antibody (Center) AP13630c
    All lanes : Anti-ASPG Antibody (Center) at 1:1000 dilution Lane 1: Human heart lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession Q86U10
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 374569
Other Names 60 kDa lysophospholipase, L-asparaginase, L-asparagine amidohydrolase, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, ASPG, C14orf76
Target/Specificity This ASPG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human ASPG.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsASPG Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ASPG
Synonyms C14orf76
Function Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.
Research Areas

BACKGROUND

ASPG exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

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