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NUP50 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NUP50 Antibody (C-term) AP1913b
    Western blot analysis of NUP50 (arrow) using rabbit polyclonal NUP50 Antibody (C-term) (RB08362). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).
  • 1 - NUP50 Antibody (C-term) AP1913b
    Western blot analysis of anti-NUP50 Pab (Cat. #AP1913b) in mouse brain tissue lysate. NUP50(arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9UKX7
Other Accession O08587, Q9JIH2, Q8N6V5
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50144 Da
Additional info
Gene ID 10762
Other Names Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L
Target/Specificity This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human NUP50.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNUP50 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NUP50
Synonyms NPAP60L
Function Component of the nuclear pore complex that has a direct role in nuclear protein import (PubMed:20016008). Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling (PubMed:20016008). Interacts with regulatory proteins of cell cycle progression including CDKN1B (By similarity). This interaction is required for correct intracellular transport and degradation of CDKN1B (By similarity).
Cellular Location Nucleus, nuclear pore complex. Nucleus membrane {ECO:0000250|UniProtKB:O08587}; Peripheral membrane protein {ECO:0000250|UniProtKB:O08587}; Nucleoplasmic side {ECO:0000250|UniProtKB:O08587}. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity) Dissociates from the NPC structure early during prophase of mitosis (PubMed:12802065). Associates with the newly formed nuclear membrane during telophase (PubMed:12802065). In the testis, the localization changes during germ cell differentiation from the nuclear surface in spermatocytes to the whole nucleus (interior) in spermatids and back to the nuclear surface in spermatozoa (By similarity). {ECO:0000250|UniProtKB:O08587, ECO:0000269|PubMed:12802065}
Tissue Location Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver
Research Areas

BACKGROUND

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.

REFERENCES

Lindsay, M.E., et al., Cell 110(3):349-360 (2002).
Swaminathan, S., et al., Dev. Cell 3(3):304-306 (2002).
Smitherman, M., et al., Mol. Cell. Biol. 20(15):5631-5642 (2000).
Guan, T., et al., Mol. Cell. Biol. 20(15):5619-5630 (2000).
Collins, J.E., et al., Genome Biol. 5 (10), R84 (2004) (): ().

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