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EHD2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - EHD2 Antibody (C-term) AP21050a
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using AP21050A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - EHD2 Antibody (C-term) AP21050a
    Immunohistochemical analysis of paraffin-embedded human heart tissue using AP21050A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - EHD2 Antibody (C-term) AP21050a
    All lanes : Anti-EHD2 Antibody (C-term) at 1:500-1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Human heart tissue lysate Lane 3: Mouse lung tissue lysate Lane 4: Rat lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - EHD2 Antibody (C-term) AP21050a
    All lanes : Anti-EHD2 Antibody (C-term) at 1:1000 dilution Lane 1: Human heart tissue lysate Lane 2: A549 whole cell lysate Lane 3: Human placenta tissuee lysate Lane 4: Hela whole cell lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q9NZN4
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 30846
Other Names EH domain-containing protein 2, PAST homolog 2, EHD2, PAST2
Target/Specificity This EHD2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 415-449 amino acids from the C-terminal region of human EHD2.
Dilution IHC-P-Leica~~1:500
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEHD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EHD2 (HGNC:3243)
Function ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity). Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914). Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed:25588833).
Cellular Location Cell membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8BH64}; Cytoplasmic side {ECO:0000250|UniProtKB:Q8BH64}. Membrane, caveola {ECO:0000250|UniProtKB:Q8BH64}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8BH64}; Cytoplasmic side {ECO:0000250|UniProtKB:Q8BH64}. Endosome membrane {ECO:0000250|UniProtKB:Q4V8H8}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q4V8H8}; Cytoplasmic side {ECO:0000250|UniProtKB:Q4V8H8}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q8BH64}. Note=Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes. {ECO:0000250|UniProtKB:Q8BH64}
Tissue Location Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle
Research Areas

BACKGROUND

Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity).

REFERENCES

Pohl U.,et al.Genomics 63:255-262(2000).
Benjamin S.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Aboulaich N.,et al.Biochem. J. 383:237-248(2004).

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