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Ihh Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Ihh Antibody (C-term) AP2704b
    All lanes: Anti-Ihh Antibody (Center) at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 49 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q80XI9
Other Accession Q91612, P97812, Q14623, Q98862, Q98938
Reactivity Human, Mouse
Predicted Chicken, Zebrafish, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49445 Da
Antigen Region 169-200 aa
Additional Information
Gene ID 16147
Other Names Indian hedgehog
Target/Specificity This Ihh antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-200 amino acids from the C-terminal region of human Ihh.
Dilution WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIhh Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ihh {ECO:0000313|EMBL:AAH46984.1, ECO:0000313|MGI:MGI:96533}
Function [Protein hedgehog N-product]: The dually lipidated hedgehog protein N-product is a morphogen which is essential for a variety of patterning events during development.
Cellular Location Endoplasmic reticulum membrane {ECO:0000256|ARBA:ARBA00004586}. Secreted {ECO:0000256|ARBA:ARBA00004613} [Protein hedgehog N-product]: Cell membrane {ECO:0000256|RuleBase:RU280812}; Lipid-anchor {ECO:0000256|RuleBase:RU280812}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ihh (Indian hedgehog) is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal.

REFERENCES

Kobayashi,T., Proc. Natl. Acad. Sci. U.S.A. 105 (6), 1949-1954 (2008)
Gat-Yablonski,G.,J. Pediatr. Endocrinol. Metab. 20 (11), 1215-1222 (2007)
Koyama,E., Ann. N. Y. Acad. Sci. 1116, 100-112 (2007)

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