NPM1 Antibody
Purified Mouse Monoclonal Antibody (Mab)
- 产品详情
- 实验流程
- 背景知识
Application ![]()
| WB |
---|---|
Primary Accession | P06748 |
Reactivity | Human, Mouse |
Host | Mouse |
Clonality | Monoclonal |
Isotype | IgG1 |
Calculated MW | 32575 Da |
Gene ID | 4869 |
---|---|
Other Names | B23;MGC104254;NMP1;NO38;NPM 1;NPM;NPM_HUMAN;NPM1;Nucleolar Phosphoprotein B23;Nucleolar protein NO38;Nucleophosmin (nucleolar phosphoprotein B23 numatrin);Nucleophosmin; Nucleophosmin/B23.2;Nucleophosmin/nucleoplasmin family member 1;Nucleoplasmin Family Member 1;Numatrin;OTTHUMP00000161024;OTTHUMP00000161025;OTTHUMP00000223397; OTTHUMP00000223398;TRK fused gene. |
Dilution | WB~~1:1000 |
Format | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.09% (W/V) sodium azide, 50%,glycerol |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | NPM1 (HGNC:7910) |
---|---|
Synonyms | NPM |
Function | Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029). May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity). |
Cellular Location | Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Co-localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis. Can shuttle between cytoplasm and nucleus (PubMed:38231884) |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.
REFERENCES
Chan W.-Y.,et al.Biochemistry 28:1033-1039(1989).
Li X.,et al.Biochem. Biophys. Res. Commun. 163:72-78(1989).
Zhang X.T.,et al.Biochem. Biophys. Res. Commun. 164:176-184(1989).
Chan P.-K.,et al.Nucleic Acids Res. 25:1225-1232(1997).
Okuwaki M.,et al.Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问,联系:0512-88856768 tech-china@abcepta.com.