CstF-64T Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Anti-CstF-64T Antibody at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q9H0L4
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	64 KDa

Additional info
Other Names	Cleavage stimulation factor subunit 2 tau variant, CF-1 64 kDa subunit tau variant, Cleavage stimulation factor 64 kDa subunit tau variant, CSTF 64 kDa subunit tau variant, TauCstF-64, CSTF2T, KIAA0689
Dilution	WB~~ 1:1000
Format	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CSTF2T
Synonyms	KIAA0689
Function	May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs (By similarity).
Cellular Location	Nucleus. EMBL; AY130299; AAN05429.1; -; mRNA EMBL; AL136747; CAB66681.1; -; mRNA EMBL; AK222574; BAD96294.1; -; mRNA EMBL; AK314318; BAG36966.1; -; mRNA EMBL; CH471083; EAW54142.1; -; Genomic_DNA EMBL; BC028239; AAH28239.1; -; mRNA EMBL; AB014589; BAA31664.1; -; mRNA CCDS; CCDS7245.1; - RefSeq; NP_056050.1; NM_015235.2 UniGene; Hs.591358; - ProteinModelPortal; Q9H0L4; - SMR; Q9H0L4; - BioGrid; 116881; 33 IntAct; Q9H0L4; 27 MINT; Q9H0L4; - STRING; 9606.ENSP00000332444; - iPTMnet; Q9H0L4; - PhosphoSitePlus; Q9H0L4; - BioMuta; CSTF2T; - DMDM; 71153234; - REPRODUCTION-2DPAGE; IPI00550906; - EPD; Q9H0L4; - jPOST; Q9H0L4; - MaxQB; Q9H0L4; - PaxDb; Q9H0L4; - PeptideAtlas; Q9H0L4; - PRIDE; Q9H0L4; - ProteomicsDB; 80293; - DNASU; 23283; - Ensembl; ENST00000331173; ENSP00000332444; ENSG00000177613 GeneID; 23283; - KEGG; hsa:23283; - UCSC; uc001jjp.4; human CTD; 23283; - DisGeNET; 23283; - EuPathDB; HostDB:ENSG00000177613.8; - GeneCards; CSTF2T; - HGNC; HGNC:17086; CSTF2T HPA; HPA039216; - HPA; HPA062021; - MIM; 611968; gene neXtProt; NX_Q9H0L4; - OpenTargets; ENSG00000177613; - PharmGKB; PA134933809; - eggNOG; KOG0108; Eukaryota eggNOG; ENOG410XQBV; LUCA GeneTree; ENSGT00940000161661; - HOGENOM; HOG000214373; - HOVERGEN; HBG051145; - InParanoid; Q9H0L4; - KO; K14407; - OMA; IQGTGMQ; - OrthoDB; 1455080at2759; - PhylomeDB; Q9H0L4; - TreeFam; TF314948; - Reactome; R-HSA-109688; Cleavage of Growing Transcript in the Termination Region Reactome; R-HSA-72163; mRNA Splicing - Major Pathway Reactome; R-HSA-72187; mRNA 3'-end processing Reactome; R-HSA-77595; Processing of Intronless Pre-mRNAs GeneWiki; CSTF2T; - GenomeRNAi; 23283; - PRO; PR:Q9H0L4; - Proteomes; UP000005640; Chromosome 10 Bgee; ENSG00000177613; Expressed in 228 organ(s), highest expression level in left coronary artery Genevisible; Q9H0L4; HS GO; GO:0005622; C:intracellular; IDA:LIFEdb GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IBA:GO_Central GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0003729; F:mRNA binding; IBA:GO_Central GO; GO:0003723; F:RNA binding; HDA:UniProtKB GO; GO:0031124; P:mRNA 3'-end processing; TAS:Reactome GO; GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome GO; GO:0098789; P:pre-mRNA cleavage required for polyadenylation; IBA:GO_Central GO; GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome Gene3D; 1.10.20.70; -; 1 Gene3D; 3.30.70.330; -; 1 InterPro; IPR038192; CSTF2_C_sf InterPro; IPR025742; CSTF2_hinge InterPro; IPR026896; CSTF_C InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf InterPro; IPR035979; RBD_domain_sf InterPro; IPR000504; RRM_dom Pfam; PF14327; CSTF2_hinge; 1 Pfam; PF14304; CSTF_C; 1 Pfam; PF00076; RRM_1; 1 SMART; SM00360; RRM; 1 SUPFAM; SSF54928; SSF54928; 1 PROSITE; PS50102; RRM; 1 1: Evidence at protein level; Complete proteome; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding CHAIN 1 616 Cleavage stimulation factor subunit 2 tau variant /FTId=PRO_0000081534 DOMAIN 16 94 RRM. {ECO:0000255\|PROSITE- ProRule:PRU00176} REPEAT 418 422 1-1 REPEAT 423 427 1-2; approximate REPEAT 428 432 1-3; approximate REPEAT 433 437 1-4; approximate REPEAT 438 442 1-5; approximate REPEAT 443 447 1-6 REPEAT 448 452 1-7; approximate REPEAT 453 457 1-8; approximate REPEAT 458 462 1-9; approximate REPEAT 505 509 2-1 REPEAT 510 514 2-2 REPEAT 515 519 2-3 REPEAT 520 524 2-4 REPEAT 525 529 2-5; approximate REPEAT 530 534 2-6 REPEAT 535 539 2-7; approximate REPEAT 540 544 2-8; approximate REPEAT 545 549 2-9; approximate REGION 418 462 9 X 5 AA tandem repeats of M-E-T-R-[AG] REGION 505 549 9 X 5 AA tandem repeats of G-[AT]-G-[MI]- Q COMPBIAS 260 556 Gly-rich MOD_RES 320 320 Phosphothreonine MOD_RES 563 563 Phosphoserine CONFLICT 22 22 N -> S (in Ref. 4; BAD96294) CONFLICT 553 553 K -> R (in Ref. 6; AAH28239) SEQUENCE 616 AA; 64437 MW; 916BB9B0802AC847 CRC64; MSSLAVRDPA MDRSLRSVFV GNIPYEATEE QLKDIFSEVG SVVSFRLVYD RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE LKSLGPAAPI IDSPYGDPID PEDAPESITR AVASLPPEQM FELMKQMKLC VQNSHQEARN MLLQNPQLAY ALLQAQVVMR IMDPEIALKI LHRKIHVTPL IPGKSQSVSV SGPGPGPGPG LCPGPNVLLN QQNPPAPQPQ HLARRPVKDI PPLMQTPIQG GIPAPGPIPA AVPGAGPGSL TPGGAMQPQL GMPGVGPVPL ERGQVQMSDP RAPIPRGPVT PGGLPPRGLL GDAPNDPRGG TLLSVTGEVE PRGYLGPPHQ GPPMHHASGH DTRGPSSHEM RGGPLGDPRL LIGEPRGPMI DQRGLPMDGR GGRDSRAMET RAMETEVLET RVMERRGMET CAMETRGMEA RGMDARGLEM RGPVPSSRGP MTGGIQGPGP INIGAGGPPQ GPRQVPGISG VGNPGAGMQG TGIQGTGMQG AGIQGGGMQG AGIQGVSIQG GGIQGGGIQG ASKQGGSQPS SFSPGQSQVT PQDQEKAALI MQVLQLTADQ IAMLPPEQRQ SILILKEQIQ KSTGAS

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs (By similarity).

REFERENCES

Dass B.,et al.Genomics 80:509-514(2002).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 2,310.00

Cat# AP53338

Size:

100 µl

Price:

¥ 2,310.00

Quantity:

reduce add

Availability: 3-5 days

询价

CstF-64T Antibody

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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