癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SARS virus PUP2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| E |
|---|---|
| Primary Accession | P59633 |
| Other Accession | NP_828853 |
| Reactivity | SARS |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Other Names | Non-structural protein 3b, ns3b, Accessory protein 3b, Protein X2, 3b |
|---|---|
| Target/Specificity | This SARS virus PUP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of SARS virus PUP2. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SARS virus PUP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | 3b |
|---|---|
| Function | Induces host cell G0/G1 arrest and apoptosis. |
| Cellular Location | Host nucleus, host nucleolus. Host mitochondrion EMBL; AY278741; AAP13447.1; -; Genomic_RNA EMBL; AY274119; AAP41039.1; -; Genomic_RNA EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY278554; AAP13569.1; -; Genomic_RNA EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY278488; AAP30032.1; -; Genomic_RNA EMBL; AY278489; AAP51229.1; -; Genomic_RNA EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY291451; AAP37019.1; -; Genomic_RNA EMBL; AY310120; AAP50487.1; -; Genomic_RNA EMBL; AY291315; AAP33699.1; -; Genomic_RNA EMBL; AY463059; AAR86789.1; -; Genomic_RNA EMBL; AY338174; AAQ01599.1; -; Genomic_RNA EMBL; AY338175; AAQ01611.1; -; Genomic_RNA EMBL; AY348314; AAP97884.1; -; Genomic_RNA EMBL; AP006557; BAC81350.1; -; Genomic_RNA EMBL; AP006558; BAC81364.1; -; Genomic_RNA EMBL; AP006559; BAC81378.1; -; Genomic_RNA EMBL; AP006560; BAC81392.1; -; Genomic_RNA EMBL; AP006561; BAC81406.1; -; Genomic_RNA EMBL; AY323977; AAP72976.1; -; Genomic_RNA EMBL; AY427439; AAQ94062.1; -; Genomic_RNA RefSeq; NP_828853.1; NC_004718.3 SMR; P59633; - PRIDE; P59633; - GeneID; 1489670; - KEGG; vg:1489670; - Proteomes; UP000000354; Genome Proteomes; UP000103670; Genome Proteomes; UP000109640; Genome Proteomes; UP000116947; Genome Proteomes; UP000121636; Genome Proteomes; UP000131569; Genome Proteomes; UP000131955; Genome Proteomes; UP000137377; Genome Proteomes; UP000138690; Genome Proteomes; UP000143093; Genome Proteomes; UP000145651; Genome Proteomes; UP000146108; Genome Proteomes; UP000146181; Genome Proteomes; UP000146296; Genome Proteomes; UP000148194; Genome Proteomes; UP000153467; Genome Proteomes; UP000160648; Genome Proteomes; UP000164441; Genome Proteomes; UP000172416; Genome GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW GO; GO:0001817; P:regulation of cytokine production; IEP:CACAO InterPro; IPR022117; SARS_3b Pfam; PF12383; SARS_3b; 1 1: Evidence at protein level; Apoptosis; Complete proteome; G0/G1 host cell cycle checkpoint dysregulation by virus; Host mitochondrion; Host nucleus; Host-virus interaction; Modulation of host cell apoptosis by virus; Modulation of host cell cycle by virus; Reference proteome CHAIN 1 154 Non-structural protein 3b /FTId=PRO_0000106132 REGION 80 138 Mitochondrial targeting signal REGION 134 154 Nucleolar targeting MOTIF 135 153 Bipartite nuclear localization signal VARIANT 31 31 K -> Q (in strain: Isolate GD01) VARIANT 99 99 T -> I (in strain: Isolate BJ02) VARIANT 115 115 L -> Q (in strain: Isolate GD01) VARIANT 121 121 Q -> P (in strain: Isolate BJ01 and Isolate BJ04) SEQUENCE 154 AA; 17750 MW; 82AB0B68F11060A8 CRC64; MMPTTLFAGT HITMTTVYHI TVSQIQLSLL KVTAFQHQNS KKTTKLVVIL RIGTQVLKTM SLYMAISPKF TTSLSLHKLL QTLVLKMLHS SSLTSLLKTH RMCKYTQSTA LQELLIQQWI QFMMSRRRLL ACLCKHKKVS TNLCTHSFRK KQVR |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The SARS-CoV genome contains five major open reading frames (ORFs) that encode the replicase polyprotein (R), the spike (S), envelope (E), and membrane (M) glycoproteins; and the nucleocapsid protein (N). Other proteins not falling into these categories have been termed PUPs (putative uncharacterized proteins) for their unknown structural or functional features and dissimilarity to those known sequences. However, it has been found that some of the PUPs matched the entries in the NCBI database.PUP2 has a counterpart in Isolate Tor2, the ORF4. It matches 4 segments of different entries in GenBank: 138 amino acids with NADH dehydrogenase subunit2 of Laudakia stoliczkana, 137 amino acids with a hypothetical protein of Methanosarcina barkeri, 85 amino acids with myosin IXb of Homo sapiens, and 85 amino acids with MY9B HUMAN myosin IXb. All of these alignments are 28% identical.
REFERENCES
He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).
Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).
Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
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