> 首页 > 产品 > 一抗 > 其他 > Aquaporin 4 Monoclonal Antibody(4H1)

Aquaporin 4 Monoclonal Antibody(4H1)

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-appendix tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-brain tissue. 1,Aquaporin 4 Monoclonal Antibody(4H1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue, diluted at 1:2000.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF
Primary Accession	P55087
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	34830 Da

Additional Information
Gene ID	361
Other Names	AQP4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4
Dilution	WB~~WB: 1:1000 IF: 1:100-200 IHC 1:50-300 IHC-P~~N/A IF~~1:50~200
Format	PBS, pH 7.4, containing 0.09% (W/V) sodium azide as Preservative and 50% Glycerol.
Storage Conditions	-20℃

Protein Information
Name	AQP4
Function	Forms a water-specific channel (PubMed:19383790, PubMed:7559426, PubMed:8601457). Plays an important role in brain water homeostasis (PubMed:37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250\|UniProtKB:P55088}; Multi-pass membrane protein. Endosome membrane {ECO:0000250\|UniProtKB:P47863}. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection {ECO:0000250\|UniProtKB:P47863}. Note=Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries {ECO:0000250\|UniProtKB:P47863}
Tissue Location	Detected in skeletal muscle (PubMed:29055082). Detected in stomach, along the glandular base region of the fundic gland (at protein level) (PubMed:8601457). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary (PubMed:7559426, PubMed:8601457).