|Application ||IHC-P, FC, WB, E|
|Reactivity||Human, Mouse, Rat|
|Other Names||Glutaredoxin-2, mitochondrial, GLRX2, GRX2|
|Target/Specificity||This GLRX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-146 amino acids from the C-terminal region of human GLRX2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GLRX2 Antibody (C-term T135) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.|
|Cellular Location||Isoform 1: Mitochondrion.|
|Tissue Location||Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.|
Provided below are standard protocols that you may find useful for product applications.
GLRX2 are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions.
Lundberg M., Johansson C. J. Biol. Chem. 276:26269-26275(2001)
Gladyshev V.N., Liu A. J. Biol. Chem. 276:30374-30380(2001)
Lillig C.H. Proc. Natl. Acad. Sci. U.S.A. 101:13227-13232(2004)