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HSPA1A/HSPA1B Antibody (Y41)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - HSPA1A/HSPA1B Antibody (Y41) AP7574a
    Immunohistochemical analysis of AP7574a on paraffin-embedded Human colon tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 14 - HSPA1A/HSPA1B Antibody (Y41) AP7574a
    Immunohistochemical analysis of AP7574a on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - HSPA1A/HSPA1B Antibody (Y41) AP7574a
    All lanes : Anti-HSPA1A(Y41) Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: C2C12 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: C6 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P08107
Other Accession P11147, P63018, P63017, P11142, Q90473, P19378, O73885, P19120, P09446, P48741, Q04967, P17066, P22202, P09435, P14659, P17156, P54652, P34933, Q07439, P02827, P08106, P55063, A5A8V7, P16627, Q4R888, P34931, P0CB32, Q6S4N2, P17879, Q27965, Q61696, Q27975
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat, Zebrafish, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine, C.Elegans, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Target/Specificity This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-48 amino acids from human HSPA1A/HSPA1B.
Dilution IHC-P~~1:100
WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSPA1A/HSPA1B Antibody (Y41) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Characterization of cadmium chloride-induced BiP accumulation in Xenopus laevis A6 kidney epithelial cells.
Author : Shirriff CS1, Heikkila JJ2.
Comp Biochem Physiol C Toxicol Pharmacol. 2016 Oct 13. pii: S1532-0456(16)30129-6. doi: 10.1016/j.cbpc.2016.10.003. [Epub ahead of print]
27746171

BACKGROUND

HSPA1A is a member of the heat shock protein 70 family. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.

REFERENCES

Mueller, T., et al., Transplantation 78(2):292-295 (2004).
Fekete, A., et al., Pediatr. Res. 54(4):452-455 (2003).
Broquet, A.H., et al., J. Biol. Chem. 278(24):21601-21606 (2003).
Bruce, C.R., et al., Diabetes 52(9):2338-2345 (2003).
Anwar, A., et al., J. Biol. Chem. 277(16):14060-14067 (2002).

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