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PMM2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - PMM2 Antibody (C-term) AW5041
    All lanes : Anti-PMM2 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession O15305
Other Accession Q9Z2M7, Q60HD6, Q3SZJ9, NP_000294.1
Reactivity Human
Predicted Mouse, Rat, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 28082 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 5373
Antigen Region 198-226 aa
Other Names PMM2; Phosphomannomutase 2
Dilution WB~~1:2000
Target/Specificity This PMM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human PMM2.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPMM2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PMM2
Function Involved in the synthesis of the GDP-mannose and dolichol- phosphate-mannose required for a number of critical mannosyl transfer reactions.
Cellular Location Cytoplasm.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The protein encoded by this gene catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate, which is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. Mutations in this gene have been shown to cause defects in glycoprotein biosynthesis, which manifests as carbohydrate-deficient glycoprotein syndrome type I. [provided by RefSeq].

REFERENCES

Vega, A.I., et al. Hum. Mutat. 30(5):795-803(2009)
Coman, D., et al. Am. J. Med. Genet. A 146(3):389-392(2008)
Vermeer, S., et al. J. Neurol. 254(10):1356-1358(2007)
Coman, D., et al. J Clin Neurosci 14(7):668-672(2007)
Schollen, E., et al. Mol. Genet. Metab. 90(4):408-413(2007)

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