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EIF1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - EIF1 Antibody (C-term) AW5308
    Western blot analysis of lysates from mouse cerebellum tissue and A549 cell line (from left to right), using EIF1 Antibody (C-term)(Cat. #AW5308). AW5308 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P41567
Reactivity Mouse, Rat, Human
Host Rabbit
Clonality Polyclonal
Calculated MW 12732 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 10209
Antigen Region 100-133 aa
Other Names Eukaryotic translation initiation factor 1, eIF1, A121, Protein translation factor SUI1 homolog, Sui1iso1, EIF1, SUI1
Dilution WB~~1:1000
Target/Specificity This EIF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 100-133 amino acids from the C-terminal region of human EIF1.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF1
Synonyms SUI1
Function Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed:12435632, PubMed:14600024, PubMed:9732867). Together with eIF1A (EIF1AX), EIF1 facilitates scanning and is essential for start codon recognition on the basis of AUG nucleotide context and location relative to the 5'-cap (PubMed:12435632, PubMed:14600024, PubMed:9732867). Participates to initiation codon selection by influencing the conformation of the 40S ribosomal subunit and the positions of bound mRNA and initiator tRNA; this is possible after its binding to the interface surface of the platform of the 40S ribosomal subunit close to the P-site (PubMed:14600024). Together with eIF1A (EIF1AX), also regulates the opening and closing of the mRNA binding channel, which ensures mRNA recruitment, scanning and the fidelity of initiation codon selection (PubMed:9732867). Continuously monitors and protects against premature and partial base-pairing of codons in the 5'-UTR with the anticodon of initiator tRNA (PubMed:12435632, PubMed:9732867). Together with eIF1A (EIF1AX), acts for ribosomal scanning, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes (PubMed:9732867). Interacts with EIF4G1, which in a mutual exclusive interaction associates either with EIF1 or with EIF4E on a common binding site (PubMed:29987188). EIF4G1-EIF1 complex promotes ribosome scanning (on both short and long 5'UTR), leaky scanning (on short 5'UTR) which is the bypass of the initial start codon, and discrimination against cap-proximal AUG (PubMed:29987188). Is probably maintained within the 43S PIC in open conformation thanks to eIF1A-EIF5 interaction (PubMed:24319994). Once the correct start codon is reached, EIF1 is physically excluded from the decoding site, shifting the PIC into the closed conformation and arresting it at the start codon (PubMed:22813744).
Cellular Location Cytoplasm.

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.

REFERENCES

Fields C.A.,et al.Biochem. Biophys. Res. Commun. 198:288-291(1994).
Singh S.K.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Sheikh M.S.,et al.J. Biol. Chem. 274:16487-16493(1999).
Mendell J.T.,et al.Mol. Cell. Biol. 20:8944-8957(2000).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).

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