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MP68 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - MP68 Antibody (C-term) AW5384
    All lanes : Anti-MP68 Antibody (C-term) at 1:1000 dilution Lane 1: CEM whole cell lysates Lane 2: human heart lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 7 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P56378
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 6662 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 9556
Antigen Region 44-78 aa
Other Names 68 kDa mitochondrial proteolipid, MP68, C14orf2
Dilution WB~~1:1000
Target/Specificity This MP68 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-78 amino acids from the C-terminal region of human MP68.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMP68 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP5MJ (HGNC:1188)
Synonyms ATP5MPL, C14orf2, MP68
Function Subunit j, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (PubMed:37244256). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel (PubMed:37244256). These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk (PubMed:37244256). During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (PubMed:37244256). Minor subunit required to maintain the ATP synthase population in the mitochondria (PubMed:24330338).
Cellular Location Mitochondrion membrane; Single-pass membrane protein
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

Mao M.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Zhang C.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Li W.B.,et al.Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Heilig R.,et al.Nature 421:601-607(2003).

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