癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide
Mouse Monoclonal Antibody [Clone TOP1MT/488 ]
- 产品详情
- 实验流程
- 背景知识
Application 
| IHC, IF, FC |
|---|---|
| Primary Accession | Q969P6 |
| Other Accession | 116447, 528574 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse / IgG2b, kappa |
| Clone Names | TOP1MT/488 |
| Calculated MW | 69872 Da |
| Gene ID | 116447 |
|---|---|
| Other Names | DNA topoisomerase I, mitochondrial, TOP1mt, 5.99.1.2, TOP1MT |
| Application Note | IHC~~1:100~500 IF~~1:50~200 FC~~1:10~50 |
| Storage | Store at 2 to 8°C.Antibody is stable for 24 months. |
| Precautions | Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody - With BSA and Azide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TOP1MT |
|---|---|
| Function | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)- enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). |
| Cellular Location | Mitochondrion. |
| Tissue Location | Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
REFERENCES
Zhang, H., Barcel�, J.M., Lee, B., Kohlhagen, G., Zimonjic, D.B., Popescu, N.C. and Pommier, Y. 2001. Human mitochondrial topoisomerase I. Proc. Natl. Acad. Sci. USA 98: 10608-10613. | Zhang, H., Meng, L.H. and Pommier, Y. 2007. Mitochondrial topoisomerases and alternative splicing of the human TOP1mt gene. Biochimie 89: 474-481
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