BMI1 Antibody

Mouse Monoclonal Antibody (Mab)

All lanes : Anti-BMI1 Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
BMI1 Antibody (Cat. #AM1930b) western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow).
Confocal immunofluorescent analysis of BMI1 Antibody (Cat#AM1930b) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

产品详情
文献引用 : 1
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Primary Accession	P35226
Other Accession	NP_005171.4
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Clone Names	282CT3.7.6

Additional Information
Other Names	Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51
Target/Specificity	This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein.
Dilution	WB~~1:500~1000 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	BMI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

REFERENCES

Ismail, I.H., et al. J. Cell Biol. 191(1):45-60(2010)
Yang, M.H., et al. Nat. Cell Biol. 12(10):982-992(2010)
Kikuchi, J., et al. Cancer 116(12):3015-3024(2010)
Honig, A., et al. Anticancer Res. 30(5):1559-1564(2010)
Venkataraman, S., et al. PLoS ONE 5 (6), E10748 (2010) :

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¥ 699.00

Cat# AM1930b

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¥ 699.00

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BMI1 Antibody

Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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